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CCL11/Eotaxin Antibody [Biotin]

 (8 Publications)
 (1 Review)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF-320-NA
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF-320-NA
Carrier Free
AF-320-NA
Carrier Free
AF-320-SP
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CCL11/Eotaxin Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human CCL11/Eotaxin (R&D Systems, Catalog # 320-EO)
Gly24-Pro97
Accession # Q6I9T4
Specificity
Detects human CCL11/Eotaxin in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant mouse (rm) CCL11, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rhMIP-1 alpha , and rhRANTES is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL11
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human CCL11/Eotaxin Antibody (Catalog # MAB320)
ELISA Detection: Human CCL11/Eotaxin Biotinylated Antibody (Catalog # BAF320)
Standard: Recombinant Human CCL11/Eotaxin (Catalog # 320-EO)
Readout System
Reviewed Applications
Read 1 Review rated 4
using
BAF320 in the following applications:
Publications
Read Publications using
BAF320 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL11/Eotaxin Antibody [Biotin]

  • C-C motif chemokine 11
  • CCL11
  • chemokine (C-C motif) ligand 11
  • Eosinophil chemotactic protein
  • Eotaxin
  • eotaxin-1
  • MGC22554
  • SCYA11Small-inducible cytokine A11
  • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1 - 3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

  1. Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
  2. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  3. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  4. Choe, H. et al. (1996) Cell 85:1135.
 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL11/Eotaxin Antibody (BAF320)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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ELISA Development
(4)
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Human
(4)
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Showing Publications 1 - 8 of 8.
Publications using BAF320 Applications Species
Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection Cell 2020-08-01 [PMID: 32645325]
Martina Sandberg Abelius, Uta Enke, Frauke Varosi, Heike Hoyer, Ekkehard Schleussner, Maria C. Jenmalm et al. Placental immune response to apple allergen in allergic mothers Journal of Reproductive Immunology 2014-12-01 [PMID: 25048667]
T. R. Abrahamsson, M. Sandberg Abelius, A. Forsberg, B. Björkstén, M. C. Jenmalm A Th1/Th2-associated chemokine imbalance during infancy in children developing eczema, wheeze and sensitization Clinical & Experimental Allergy 2011-12-01 [PMID: 21801246]
Catrin Furuhjelm, Maria C Jenmalm, Karin Fälth-Magnusson, Karel Duchén Th1 and Th2 Chemokines, Vaccine-Induced Immunity, and Allergic Disease in Infants After Maternal omega -3 Fatty Acid Supplementation During Pregnancy and Lactation Pediatric Research 2011-03-01 [PMID: 21099447]
Osei-Kumah A, Smith R, Clifton VL Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma. Cytokine, 2008-07-17;43(2):187-93. 2008-07-17 [PMID: 18639465] (ELISA Development, Human) ELISA Development Human
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006-06-16;120(2):220-31. 2006-06-16 [PMID: 16782408] (ELISA Development, Human) ELISA Development Human
Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission. Parasitology, 2005-03-01;130(0):333-42. 2005-03-01 [PMID: 15796016] (ELISA Development, Human) ELISA Development Human
Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP Chemoattractant factors in breast milk from allergic and nonallergic mothers. Pediatr. Res., 2000-05-01;47(5):592-7. 2000-05-01 [PMID: 10813582] (ELISA Development, Human) ELISA Development Human

Review for CCL11/Eotaxin Antibody (BAF320) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using BAF320:
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  4
reviewed by:
Verified Customer
 ELISA Human 01/13/2015
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Summary

ApplicationELISA
Sample TestedSee PMID 21801246
SpeciesHuman

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 Verified Customer
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Application: ELISA
Species: Human
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Bioinformatics

Gene Symbol CCL11
Uniprot