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CCL11/Eotaxin Antibody (43915) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
43915
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC3201U-100UG
Alexa Fluor 405
IC3201V-100UG
Alexa Fluor 532
FAB3201X
Alexa Fluor 594
IC3201T-100UG
Alexa Fluor 647
IC3201R-100UG
Alexa Fluor 700
IC3201N-100UG
Alexa Fluor 750
IC3201S-100UG
Allophycocyanin
FAB3201A
Biotin
FAB3201B
DyLight 350
FAB3201D
DyLight 405
FAB3201E
DyLight 488
FAB3201K
DyLight 550
FAB3201L
DyLight 594
FAB3201M
DyLight 650
FAB3201W
DyLight 680
FAB3201Y
DyLight 755
FAB3201Z
FITC
FAB3201F
HRP
FAB3201H
Janelia Fluor 525
FAB3201JF525
Janelia Fluor 549
FAB3201I
Janelia Fluor 646
FAB3201J
Janelia Fluor 669
FAB3201JF669
PE
FAB3201P
PerCP
FAB3201C
Unconjugated
MAB3201
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB3201
Carrier Free
MAB3201
Carrier Free
MAB3201-SP
Carrier Free
IC3201V-100UG
Carrier Free
IC3201U-100UG
Carrier Free
IC3201T-100UG
Carrier Free
IC3201S-100UG
Carrier Free
IC3201R-100UG
Carrier Free
IC3201N-100UG
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CCL11/Eotaxin Antibody (43915) [Alexa Fluor® 488] Summary

Immunogen
E. coli-derived recombinant human CCL11/Eotaxin
Specificity
Detects human CCL11/Eotaxin in Western blots. Does not cross-react with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 3, 4, 5, 8, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma , 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL11
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL11/Eotaxin Antibody (43915) [Alexa Fluor® 488]

  • C-C motif chemokine 11
  • CCL11
  • chemokine (C-C motif) ligand 11
  • Eosinophil chemotactic protein
  • Eotaxin
  • eotaxin-1
  • MGC22554
  • SCYA11Small-inducible cytokine A11
  • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1-3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

  1. Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
  2. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  3. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  4. Choe, H. et al. (1996) Cell 85:1135.
 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCL11