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Cathepsin H Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Cathepsin H Antibody [NBP1-90273] - Staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: Cathepsin H Antibody [NBP1-90273] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Cathepsin H Antibody [NBP1-90273] - Staining of human lung shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Cathepsin H Antibody [NBP1-90273] - Staining in human lung and skeletal muscle tissues using anti-CTSH antibody. Corresponding CTSH RNA-seq data are presented ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Cathepsin H Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LPSQAFEYILYNKGIMGEDTYPYQGKDGYCKFQPGKAIGFVKDVANITIYDEEAMVEAVALYNPVSFAFEVTQDFMMYRTGIYSSTSCHKTPDKVNHAVLAVGYGEKNGIPYWIVKNSWGPQWGMNGYFL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTSH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Cathepsin H Recombinant Protein Antigen (NBP1-90273PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cathepsin H Antibody

  • ACC-4
  • ACC-5
  • aleurain
  • cathepsin B3
  • cathepsin BA
  • Cathepsin H
  • CPSB
  • CTSH
  • DKFZp686B24257
  • EC 3.4.22
  • EC 3.4.22.16
  • MGC1519
  • minichain
  • N-benzoylarginine-beta-naphthylamide hydrolase

Background

Cathepsin H is encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CTSH