Caspase-9 Antibody

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-9 polyclonal antisera recognizes the proform of caspase-9 (approx. 50 kDa), and the large (approx. 35 kDa) and small (approx. 15 kDa) subunits of active/cleaved Caspase-9.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

Showing Publications 1 - 10 of 10.

Publications using NB100-56119	Applications	Species
Prasenjit Manna, Saikat Dewanjee, Swarnalata Joardar, Pratik Chakraborty, Hiranmoy Bhattacharya, Shrestha Bhanja, Chiranjib Bhattacharyya, Manas Bhowmik, Shovonlal Bhowmick, Achintya Saha, Joydeep Das, Parames C Sil Carnosic acid attenuates doxorubicin-induced cardiotoxicity by decreasing oxidative stress and its concomitant pathological consequences. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2022-07-19 [PMID: 35675861]
Liu Z, Liu Q, Zhang B et al. Blood-Brain Barrier Permeable and NO-Releasing Multifunctional Nanoparticles for Alzheimer's Disease Treatment: Targeting NO/cGMP/CREB Signaling Pathways Journal of medicinal chemistry 2021-09-23 [PMID: 34517696]
Thomas A, Samykutty A, Gomez-Gutierrez JG et al. Actively Targeted Nanodelivery of Echinomycin Induces Autophagy-Mediated Death in Chemoresistant Pancreatic Cancer In Vivo Cancers (Basel) 2020-08-14 [PMID: 32823919] (WB, Human)	WB	Human
Taggart K, Estrada A, Thompson P et al. PDK4 Deficiency Induces Intrinsic Apoptosis in Response to Starvation in Fibroblasts from Doberman Pinschers with Dilated Cardiomyopathy. Biores Open Access 2017-12-01 [PMID: 29285418] (ICC/IF, Canine)	ICC/IF	Canine
Huang X, Huang R, Gou S et al. Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action Bioconjug. Chem. 2016-08-15 [PMID: 27494235] (WB, Human)	WB	Human
Cheng Tc, Lai Cs, Chung Mc et al. Potent anti-cancer effect of 3'-hydroxypterostilbene in human colon xenograft tumors PLoS OnE et al. 2014-11-13 [PMID: 25389774] (WB, Human) Details: Caspase-9 antibody used in WB for the detection of pro and cleaved forms of Caspase 9 protein in lysates of COLO205 cancer cells treated or not with Pterostilbene and 3'-hydroxypterostilbene (Figure 2C)	WB	Human
Buneker CK, Yu R, Deedigan L et al. IFN-[gamma] combined with targeting of XIAP leads to increased apoptosis-sensitisation of TRAIL resistant pancreatic carcinoma cells. Cancer Letters. 2011-11-02 [PMID: 22104728]
Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004-10-01 [PMID: 15380478] (WB, IHC-P, Canine, Mouse, Rat) Details: Antibodies cited: 1. Caspase-9 (Active/Cleaved), IMG-5705: WB: Fig 1A (recombinant human caspase-9), Fig 4A (recombinant human caspase-9, rat brain), Fig 4B-F (isolated mitochondria from rat liver or heart). Fig 5 (PC12 cells) IHC (P): Fig 2d-k (rat kidne	WB, IHC-P	Canine, Mouse, Rat
Knoblach SM, Nikolaeva M, Huang X et al. Multiple caspases are activated after traumatic brain injury: evidence for involvement in functional outcome. J Neurotrauma. 2002-10-01 [PMID: 12427325]
Krajewski S, Krajewska M, Ellerby LM et al. Release of caspase-9 from mitochondria during neuronal apoptosis and cerebral ischemia. Proc Natl Acad Sci U S A. 1999-05-11 [PMID: 10318956]

Array NB100-56119

• Caspase-9 Antibodies
• Caspase-9 ELISA Kits
• Caspase-9 Lysate
• Caspase-9 Proteins