Home » CARD9 » CARD9 Antibodies » CARD9 Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

CARD9 Antibody [Unconjugated]

 Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CARD9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5248) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CARD9 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CARD9 Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5248-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CARD9 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CARD9
Lys225-Gln359
Accession # Q9H257
Specificity
Detects human CARD9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) CARD6, rhCARD8, rhCARD11, rhCARD14, and rhCARD10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CARD9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CARD9 Antibody [Unconjugated]

  • CANDF2
  • CARD9
  • caspase recruitment domain family, member 9
  • caspase recruitment domain-containing protein 9
  • hCARD9

Background

CARD9 (caspase recruitment domain-containing protein 9) is a 70 kDa member of the CARD module family of proteins. It is expressed in myeloid lineage cells such as macrophages, neutrophils and dendritic cells and mediates activation of p38 and Jnk, as well as NF-kappa B by interacting with BCL10 and MALT1 in a PKC-independent manner. Human CARD9 is 536 amino acids (aa) in length. It contains one CARD domain (aa 6‑98), two coiled-coil regions (aa 117‑277 and 332‑419) and a phosphorylation site at Ser460. CARD9 dimerizes with itself and BCL10. Multiple potential splice variants are noted for CARD9. There are 35 aa and 39 aa substitutions for aa 479‑536, a seven aa substitution for aa 360‑536 and an eleven aa substitution for aa 482‑536. Over aa 9‑256, human CARD9 is 88% aa identical to mouse CARD9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Dectin-1/CLEC7A
Antibody (259931)
MAB1859
Immunocytochemistry Dectin-1/CLEC7A Antibody (259931)
(2 Reviews)
(34 Publications)
Species: Hu
Applications: Block, CyTOF-ready, Flow, ICC
SYK Antibody
NBP1-32945
Western Blot SYK Antibody
Western Blot SYK Antibody
Immunoprecipitation SYK Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
Bcl-10 Antibody
(OTI4A8)
NBP2-02597
Flow Cytometry Bcl-10 Antibody (OTI4A8)
Western Blot Bcl-10 Antibody (OTI4A8)
Immunohistochemistry-Paraffin Bcl-10 Antibody (OTI4A8)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
IL-17/IL-17A
[Biotin]
DY421
N/A IL-17/IL-17A [Biotin]
(191 Publications)
Species: Mu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
CARD11/CARMA1
Antibody (8N4C9)
NBP3-16849
Immunohistochemistry-Paraffin CARD11/CARMA1 Antibody (8N4C9)
Immunohistochemistry-Paraffin CARD11/CARMA1 Antibody (8N4C9)
Immunohistochemistry CARD11/CARMA1 Antibody (8N4C9)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
STAT3 Antibody
(232209) [Unconjugate
MAB1799
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
Western Blot STAT3 Antibody (232209) [Unconjugated]
Knockout Validated STAT3 Antibody (232209) [Unconjugated]
(23 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IP, ICFlow, KO, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(667 Publications)
Species: Mu
Applications: ELISA
TLR2 Antibody
(TL2.1) - BSA Free
NB100-56722
Flow (Cell Surface) TLR2 Antibody (TL2.1) - BSA Free
Flow Cytometry TLR2 Antibody (TL2.1) - BSA Free
Block/ Neutralize TLR2 Antibody (TL2.1) - BSA Free
(64 Publications)
Species: Ca, Hu, Mu, Rb, Rt
Applications: B/N, DB, ELISA, Flow-CS, Flow, Func, ICC/IF, IP, In vitro, WB
MALT1 Antibody
NB100-56158
Western Blot MALT1 Antibody
Immunohistochemistry-Paraffin MALT1 Antibody
Species: Hu
Applications: IHC, IHC-P, IP, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(302 Publications)
Species: Mu
Applications: ELISA
Dectin-2/CLEC6A
Antibody (3D1) - BSA ...
NBP2-27159
Flow Cytometry Dectin-2/CLEC6A Antibody (3D1) - BSA Free
Immunohistochemistry-Paraffin Dectin-2/CLEC6A Antibody (3D1) - BSA Free
Western Blot Dectin-2/CLEC6A Antibody (3D1) - BSA Free
Species: Hu, Pm
Applications: CyTOF-ready, Flow-CS, Flow, IHC, IHC-P, WB
IL-23A/IL-23 P19
[Unconjugated]
1290-IL
SEC-MALS IL-23A/IL-23 P19 [Unconjugated]
(115 Publications)
Species: Hu
Applications: BA
IL-22
[Unconjugated]
782-IL/CF
SDS-PAGE IL-22 [Unconjugated]
Bioactivity IL-22 [Unconjugated]
(65 Publications)
Species: Hu
Applications: BA
IL-17F [Biotin]
DY1335B
N/A IL-17F [Biotin]
N/A IL-17F [Biotin]
(13 Publications)
Species: Hu
Applications: ELISA
Doublecortin
Antibody
NBP1-72042
Western Blot Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
(5 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
IL-23R Antibody
NB600-1147
Simple Western IL-23R Antibody
Western Blot IL-23R Antibody
Western Blot IL-23R Antibody
(1 Review)
(8 Publications)
Species: Hu, Mu
Applications: Flow-CS, Flow, IHC, IHC-P, Simple Western, WB
TLR4 Antibody
(76B357.1) - BSA Free
NB100-56566
Dual RNAscope ISH-IHC TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
(5 Reviews)
(141 Publications)
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB

Publications for CARD9 Antibody (AF5248) (0)

There are no publications for CARD9 Antibody (AF5248).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CARD9 Antibody (AF5248) (0)

There are no reviews for CARD9 Antibody (AF5248). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CARD9 Antibody (AF5248) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CARD9 Products

Blogs on CARD9

There are no specific blogs for CARD9, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Effects of SDF-1-CXCR4/CXCR7 pathway on MSC chemotaxis in vitro.(A) The chemotaxis in response to SDF-1 alpha (10 ng/ml for 12 h) was performed in the NP-MSCs and HP-MSCs treated with a neutralizing anti-CXCR4 antibody, an anti-CXCR7 antibody, and the respective isotype-matched control antibodies. *P<0.05, vs NP-MSCs; †P<0.05, vs the respective isotype-matched control antibodies. (B) NP-MSCs were transiently overexpressed with CXCR4 using pORF9-mCXCR4 vector or with CXCR7 using pORF9-mCXCR7 vector (n = 6). A negative control empty (pORF9-MCS) vector was used. (C) The transfected cells were subjected to chemotaxis in response to the indicated concentrations of SDF-1 alpha for 12 h. *P<0.05, vs the empty vector. (D and E) Western blot analysis (D) and FCM (E) were performed to determine the intracellular and extracellular expression of both CXCR4 and CXCR7 in the cells treated with or without SDF-1 alpha (50 ng/ml for 60 min). (F) The chemotaxis in response to SDF-1 alpha was performed in the cells treated with or without SDF-1 alpha . Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22511954), licensed under a CC-BY license. Not internally tested by R&D Systems.
Sheep IgG Isotype Control [Unconjugated]
5-001-A
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CARD9 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CARD9
Uniprot