Capicua Antibody - BSA Free Summary
Immunogen |
The epitope recognized by this antibody maps to a region within residue 1150-1200 of human Capicua. [NP_055940.3] |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
CIC |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:500
- Immunoprecipitation 2-5 ug/mg lysate
|
Application Notes |
This Capicua antibody is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. It is not recommended for Western blot analysis. With ICC/IF nuclear staining was observed in Neuro2A cells. |
Theoretical MW |
164 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.02% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for Capicua Antibody - BSA Free
Background
Capicua is a transcriptional repressor protein belonging to HMG-box proteins which has been suggested to play a key role in CNS development and is expressed in foetal brain as well as medulloblastomas. Capicua protein expression levels are subject to posttranscriptional regulation by signaling downstream of two RTKs - EGFR and Torso (that propagate their signals through Ras-Raf-MAPK signaling cascade) and MAPK-mediated phosphorylation promotes capicua degradation. Capicua has been shown to act as a transcriptional repressor through interaction with a co-repressor Groucho. Moreover, Bantam which is a Hippo pathway player has recently been shown to acts in a feedback loop to limit Capicua expression for regulating EGFR signaling. Capicua is well conserved in mammals, and mediates RTK-dependent responses that are linked to cell proliferation, cell differentiation and cancer. Capicua can interact with ATXN1 as well as ATXN1L, and has been shown to be involved in Spinocerebellar Ataxia Type 1 (SCA-1).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
View specific protocols for Capicua Antibody (NB110-59905):
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Video Protocols
FAQs for Capicua Antibody (NB110-59905). (Showing 1 - 1 of 1 FAQ).
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Does this antibody work in Drosophila or Zebrafish?
- I have checked the % homology between human capicua (which is what the Ab is raised to) and between drosophilia and Zebrafish capicua. The % homoloy with Drosophilia is only 27 % and our lab only recommends use if the % homology is 85% or above. The % homology with Zebrafish is only 42% so I would not recommend this either. Unfortunately all of our Capicua Anitbodies recognize the human protein therefore we do not have an antibody that would recognize Zebrafish or Drosophila Capicua.
Secondary Antibodies
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Isotype Controls
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