![Western Blot: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464] - Western blot analysis of extracts of various cell lines, using Cadherin-6/KCAD Rabbit mAb (NBP3-16464) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cadherin-6-KCAD-Antibody-5N2K3-Western-Blot-NBP3-16464-img0002.jpg "Western Blot: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464] - Western blot analysis of extracts of various cell lines, using Cadherin-6/KCAD Rabbit mAb (NBP3-16464) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | 5N2K3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 691-790 of human Cadherin-6/KCAD (P55285). DIVPEALFLPRRTPTARDNTDVRDFINQRLKENDTDPTAPPYDSLATYAYEGTGSVADSLSSLESVTTDADQDYDYLSDWGPRFKKLADMYGGVDSDKDS |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CDH6 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol, 0.05% BSA |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
![Cadherin-8 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Cadherin-8 [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | CDH6 |
![Western Blot: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464] - Western blot analysis of extracts of various cell lines, using Cadherin-6/KCAD Rabbit mAb (NBP3-16464) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cadherin-6-KCAD-Antibody-5N2K3-Western-Blot-NBP3-16464-img0001.jpg "Western Blot: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464] - Western blot analysis of extracts of various cell lines, using Cadherin-6/KCAD Rabbit mAb (NBP3-16464) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunoprecipitation: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug Cadherin-6/KCAD antibody (NBP3-16464). Western blot was performed from the immunoprecipitate using Cadherin-6/KCAD antibody (NBP3-16464) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Cadherin-6-KCAD-Antibody-5N2K3-Immunoprecipitation-NBP3-16464-img0003.jpg "Immunoprecipitation: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug Cadherin-6/KCAD antibody (NBP3-16464). Western blot was performed from the immunoprecipitate using Cadherin-6/KCAD antibody (NBP3-16464) at a dilition of 1:1000.")
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![Flow Cytometry M-Cadherin/Cadherin-15 Antibody [Unconjugated]](https://images.novusbio.com/images2/M-Cadherin_AF4096_Flow_Cytometry_8228.jpg)
![Western Blot M-Cadherin/Cadherin-15 Antibody [Unconjugated]](https://images.novusbio.com/images2/M-Cadherin_AF4096_Western_Blot_9440.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")