| Reactivity | HuSpecies Glossary |
| Applications | Bioactivity |
| Format | Carrier-Free |
| Details of Functionality | Measured by the ability of the immobilized protein to support the adhesion of Y‑79 human retinoblastoma cells. When 1 x 105 cells/well are added to Recombinant Human Protocadherin-10 coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.6-2.4 μg/mL. Optimal dilutions should be determined by each laboratory for each application. |
| Source | Mouse myeloma cell line, NS0-derived human Protocadherin-10 protein Gln19-Thr715, a substitution of Leu405Pro, with a C-terminal 6-His tag |
| Accession # | |
| N-terminal Sequence | No results obtained: Gln19 predicted |
| Protein/Peptide Type | Recombinant Proteins |
| Gene | PCDH10 |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
|
| Theoretical MW | 76.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE | 85-95 kDa, reducing conditions |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Reconstitution Instructions | Reconstitute at 200 μg/mL in PBS. |
Protocadherin 10 (PCDH10; previously OL-PCDH in mouse) is a 140 kDa protein belonging to the δ2 subgroup of protocadherins, type I transmembrane proteins that exhibit calcium-dependent but relatively weak homophilic adhesion (1, 2). Like other δ2 protocadherins, PCDH10 cDNA encodes a signal sequence (amino acids (aa) 1 ‑ 18), six cadherin domains of about 100 aa each (aa 19 ‑ 715), a transmembrane sequence (aa 716 ‑ 736), and a cytoplasmic domain (aa 737 ‑ 1040) (1). Typical of protocadherins, human PCDH10 has an alternately spliced 896 aa form that diverges at aa 878 within the C-terminal cytoplasmic domain (1). Within the extracellular domain, human PCDH10 shares 98% and 99% aa sequence identity with mouse and bovine PCDH10, respectively. PCDH10 is widely expressed, but especially prominent in discrete sections of the brain such as striatal axons in the developing ventral telencephalon and thalamocortical projections, cerebellum, visual, olfactory and limbic system neurons, and is most strongly expressed in synapse-rich regions (1 ‑ 3). It is postulated to play a role in elongation of striatal axons and neural circuit formation in the ventral telencephalon (1 ‑ 4). In many carcinomas and lymphomas, the gene is silenced by methylation (5 ‑ 8). Ectopic PCDH10 expression suppresses tumor cell growth, migration and invasion in vitro while promoting apoptosis (5, 8).
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