c-Myc Antibody (9E10) - Chimeric - Azide and BSA Free

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N., . . . Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Drosophila, Yeast.

We have publications tested in 4 applications: ChIP, ICC/IF, IF/IHC, WB.

Showing Publications 1 - 8 of 8.

Publications using NBP2-52636	Applications	Species
Liu C, Ren Q, Deng J et al. c-MYC/METTL3/LINC01006 positive feedback loop promotes migration, invasion and proliferation of non-small cell lung cancer Biomedical Journal 2023-09-27 [PMID: 37774794] (ChIP, Human)	ChIP	Human
Croft T, Venkatakrishnan P, Raj CJT et al. N-terminal protein acetylation by NatB modulates the levels of Nmnats, the NAD+ biosynthetic enzymes in Saccharomyces cerevisiae bioRxiv (WB, Yeast)	WB	Yeast
Papadas A, Deb G, Cicala A et al. Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment Cell reports 2022-08-16 [PMID: 35977482] (WB)	WB
Wang J, Xu L, Lin W et al. Single-cell transcriptome analysis reveals the immune heterogeneity and the repopulation of microglia by Hif1 alpha in mice after spinal cord injury Cell death & disease 2022-05-03 [PMID: 35504882] (IF/IHC, Mouse)	IF/IHC	Mouse
Certel SJ, McCabe BD, Stowers RS. Et al. A conditional GABAergic synaptic vesicle marker for Drosophila J Neurosci Methods 2022-02-27 [PMID: 35219770] (IF/IHC, Drosophila) Details: Citation using the Janelia Fluor 549 version of this antibody.	IF/IHC	Drosophila
Antonio-Andres G, Martinez-Ruiz GU, Morales-Martinez M et al. Transcriptional Regulation of yin-yang 1 Expression through the Hypoxia Inducible Factor-1 in Pediatric Acute Lymphoblastic Leukemia International journal of molecular sciences 2022-02-02 [PMID: 35163649] (ICC/IF, Human)	ICC/IF	Human
Croft, T, Venkatakrishnan, P Et al. N-terminal protein acetylation by NatB modulates the levels of Nmnats, the NAD+ biosynthetic enzymes in Saccharomyces cerevisiae. J Biol Chem 2020-05-22 [PMID: 32299909] (WB, Rat)	WB	Rat
Tison K. V, McKinney H. M, et al. Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection Using Drosophila vAChT. G3 (Bethesda) 2020-02-06 [PMID: 31767639] (IF/IHC, Drosophila)	IF/IHC	Drosophila

Array NBP2-52636

• c-Myc Antibodies
• c-Myc ELISA Kits
• c-Myc Lysate
• c-Myc Proteins