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Bub3 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

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Bub3 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human bub3. The immunogen is located within the last 50 amino acids of Bub3. Amino Acid Squence: SYMYEMDDTEHPEDG
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BUB3
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Peptide affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Bub3 Antibody [CoraFluor™ 1]

  • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
  • BUB3 budding uninhibited by benzimidazoles 3 homolog
  • Bub3
  • BUB3L
  • budding uninhibited by benomyl
  • budding uninhibited by benzimidazoles 3 homolog (yeast)
  • hBUB3
  • mitotic checkpoint component
  • mitotic checkpoint protein BUB3

Background

The mitotic checkpoint protein Bub3 appears to be essential during normal mitotic progression, preventing premature sister chromatid separation, missegreation and aneuploidy. Bub3 is also important during G2 and early mitosis stages, promoting normal mitotic entry. Loss of Bub3 function causes slower progression through prophase, apparently due to mitotic cyclins A and B not being accumulated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bub3 Antibody (NBP1-76796CL1) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Bub3 Antibody (NBP1-76796CL1)

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Bioinformatics

Gene Symbol BUB3