![Western Blot: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/BRM-Antibody-NB100-55309-img0003.jpg "Western Blot: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP, QCWB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1325 and 1375 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 using the numbering given in entry CAA51407.1 (GeneID 6595) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SMARCA2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SMARCA2 |
| Entrez |
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| Uniprot |
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![Immunoprecipitation: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. For blotting immunoprecipitated SMARCA2/BRM, NB100-55309 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunoprecipitation-NB100-55309-img0004.jpg "Immunoprecipitation: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. For blotting immunoprecipitated SMARCA2/BRM, NB100-55309 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.")
![QcWestern: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BRM-Antibody-QcWestern-NB100-55309-img0005.jpg "QcWestern: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")