![Western Blot: BRM Antibody (HL1115) [NBP3-13725] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRM antibody [HL1115] (NBP3-13725) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BRM-Antibody-HL1115-Western-Blot-NBP3-13725-img0001.jpg "Western Blot: BRM Antibody (HL1115) [NBP3-13725] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRM antibody [HL1115] (NBP3-13725) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1115 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human BRM. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SMARCA2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC/IF, IHC-P- Assay depenendent |
| Theoretical MW | 18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SMARCA2 |
![Immunocytochemistry/Immunofluorescence: BRM Antibody (HL1115) [NBP3-13725] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCA2 stained by SMARCA2 antibody [HL1115] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.](http://images.novusbio.com/fullsize/BRM-Antibody-HL1115-Immunocytochemistry-Immunofluorescence-NBP3-13725-img0004.jpg "Immunocytochemistry/Immunofluorescence: BRM Antibody (HL1115) [NBP3-13725] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCA2 stained by SMARCA2 antibody [HL1115] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: BRM Antibody (HL1115) [NBP3-13725] - Mouse brain. BRM stained by BRM antibody [HL1115] (NBP3-13725) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BRM-Antibody-HL1115-Immunohistochemistry-Paraffin-NBP3-13725-img0002.jpg "Immunohistochemistry-Paraffin: BRM Antibody (HL1115) [NBP3-13725] - Mouse brain. BRM stained by BRM antibody [HL1115] (NBP3-13725) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![BRM Antibody (HL1115) [NBP3-13725] - Rat brain. SMARCA2 stained by SMARCA2 antibody [HL1115] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/BRM-Antibody-HL1115-NBP3-13725-img0003.jpg "BRM Antibody (HL1115) [NBP3-13725] - Rat brain. SMARCA2 stained by SMARCA2 antibody [HL1115] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")