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Brachyury Antibody [Unconjugated]

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Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified ...read more
Western blot shows lysates of NCI‑H460 human large cell lung carcinoma cell line and MCF 10A human breast epithelial cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat ...read more
Brachyury was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) for 3 hours at room ...read more
Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 ...read more
Brachyury was detected in immersion fixed frozen sections of embryonic mouse notochord (E9.5) using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) overnight at 4 ...read more
Mesoderm-differentiated BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Brachyury/DNA complexes were immunoprecipitated using 5 μg Goat ...read more
NCI-H460 cells were stained with Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), ...read more
Generation and characterization of systemic lupus erythematosus (SLE)‐specific human‐induced pluripotent stem cells (hiPSCs). (A) Dermal fibroblasts derived from patient with SLE were reprogrammed into iPSCs using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, ChIP, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Brachyury Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Brachyury
Ser2-Glu202
Accession # O15178
Specificity
Detects human Brachyury in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
T
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1-1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF2085 in the following applications:

Publications
Read Publications using
AF2085 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

BG01V cells are licensed from ViaCyte, Inc.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody [Unconjugated]

  • brachyury protein
  • Brachyury
  • MGC104817
  • Protein T
  • SAVA
  • T brachyury (mouse) homolog
  • T brachyury homolog
  • T Brachyury Transcription Factor
  • T, brachyury homolog (mouse)
  • T-Box Transcription Factor T
  • TBXT
  • TF
  • TFT

Background

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Brachyury Antibody (AF2085)(262)

We have publications tested in 6 confirmed species: Human, Mouse, Chicken, Cynomolgus Monkey, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Xenograft.

We have publications tested in 14 applications: ChIP, Flow Cytometry, ICC, ICC/IF, IF/ICC, IHC, IHC-Fr, IHC-P, IHC/IF, Immunocytochemistry, Immunohistochemistry, Immunolocalization, In Vivo, Western Blot.


Filter By Application
ChIP
(2)
Flow Cytometry
(34)
ICC
(102)
ICC/IF
(1)
IF/ICC
(1)
IHC
(24)
IHC-Fr
(2)
IHC-P
(5)
IHC/IF
(1)
Immunocytochemistry
(15)
Immunohistochemistry
(9)
Immunolocalization
(1)
In Vivo
(1)
Western Blot
(8)
All Applications
Filter By Species
Human
(137)
Mouse
(34)
Chicken
(3)
Cynomolgus Monkey
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 262. Show All 262 Publications.
Publications using AF2085 Applications Species
McCreery, KP;Stubb, A;Stephens, R;Fursova, NA;Cook, A;Kruse, K;Michelbach, A;Biggs, LC;Keikhosravi, A;Nykänen, S;Hydén-Granskog, C;Zou, J;Lackmann, JW;Niessen, CM;Vuoristo, S;Miroshnikova, YA;Wickström, SA; Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells bioRxiv : the preprint server for biology 2024-09-07 [PMID: 39372762] (Immunohistochemistry, Immunocytochemistry, Human) Immunohistochemistry, Immunocytochemistry Human
Despin-Guitard, E;Rosa, VS;Plunder, S;Mathiah, N;Van Schoor, K;Nehme, E;Merino-Aceituno, S;Egea, J;Shahbazi, MN;Theveneau, E;Migeotte, I; Non-apical mitoses contribute to cell delamination during mouse gastrulation Nature communications 2024-08-28 [PMID: 39198421] (Immunohistochemistry, Immunocytochemistry, Mouse) Immunohistochemistry, Immunocytochemistry Mouse
Sun, J;Ren, L;Canel Rivero, G;Xu, L;Ladabaum, U;C Wu, J; Generation of two induced pluripotent stem cell lines from patients with Li-Fraumeni Syndrome carrying TP53 mutation Stem cell research 2024-08-03 [PMID: 39133963] (Immunocytochemistry, Human) Immunocytochemistry Human
Qin, H;Yu, Y;Ye, S;Alonzo, M;Garg, V;Zhao, MT; Generation of an induced pluripotent stem cell line (NCHi016-A) from a 5-year-old female with pulmonary atresia with intact ventricular septum and one-and-half ventricle palliation Stem cell research 2024-08-05 [PMID: 39126918] (Immunocytochemistry, Human) Immunocytochemistry Human
Bianchini, L;Sieber, L;Hammad, R;Schäfer, R;Kutscher, LM; Generation of two isogenic patient-derived human-induced pluripotent stem cell clones with 6q27 deletion Stem cell research 2024-10-01 [PMID: 39106599] (Immunocytochemistry, Human) Immunocytochemistry Human
Tang, P;Keshi, E;Wilken, S;Wutsdorff, L;Mougnekabol, J;Pratschke, J;Sauer, IM;Haep, N; Generation of an induced pluripotent stem cell (iPSC) line (EXSURGi001-A) from a patient homozygous for the p.Ala165Thr mutation in the MTARC1 gene Stem cell research 2024-10-01 [PMID: 39094505] (Immunohistochemistry, Human) Immunohistochemistry Human
Jo, K;Liu, ZY;Patel, G;Yu, Z;Yao, L;Teague, S;Johnson, C;Spence, J;Heemskerk, I; Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids bioRxiv : the preprint server for biology 2024-07-11 [PMID: 39026750] (Immunocytochemistry, Human) Immunocytochemistry Human
Sekulovski, N;Carleton, AE;Rengarajan, AA;Lin, CW;Juga, LN;Whorton, AE;Schmidt, JK;Golos, TG;Taniguchi, K; Temporally resolved single cell transcriptomics in a human model of amniogenesis bioRxiv : the preprint server for biology 2024-07-12 [PMID: 39026707] (Immunocytochemistry, Human) Immunocytochemistry Human
Guillot, C;Djeffal, Y;Serra, M;Pourquié, O; Control of epiblast cell fate by mechanical cues bioRxiv : the preprint server for biology 2024-06-25 [PMID: 38979228] (Immunohistochemistry, Chicken) Immunohistochemistry Chicken
Juchem, M;Lehmann, N;Behrens, YL;Bär, C;Thum, T;Hoepfner, J; CRISPR/Cas9-based GLA knockout to generate the female Fabry disease human induced pluripotent stem cell line MHHi001-A-15 Stem cell research 2024-06-20 [PMID: 38905814] (Flow Cytometry, Human) Flow Cytometry Human
Show All 262 Publications.

Reviews for Brachyury Antibody (AF2085) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using AF2085:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Mouse 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24319666
SpeciesMouse
  3
reviewed by:
Verified Customer
IF Human 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

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Recent Reviews

4
5
1
4
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3
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1
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Verified Customer
01/06/2015
Application: IF
Species: Mouse

 
Verified Customer
01/06/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol T
Uniprot