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Brachyury Antibody [NL557]

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Brachyury was detected in E9.5 mouse embryo sections using Human Brachyury NL557 conjugated Antibody (Catalog# NL2085R). Embryos were fixed in 4% paraformaldehyde, and blocked with PBS containing 10% normal donkey serum, 0.1% Triton® X 100, and 1% BSA. After blocking, sections were incubated with NL557 conjugated antibody at a final concentration of 1X (1:10 dilution) in blocking buffer for 3 hours at room temperature in the dark. Between each step, cells were washed with PBS containing BSA.
Brachyury was detected in E9.5 mouse embryo sections using Human Brachyury NL557 conjugated Antibody (Catalog# NL2085R). Embryos were fixed in 4% paraformaldehyde, and blocked with PBS containing 10% normal donkey ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
NL557

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 488
IC2085G
Allophycocyanin
IC2085A
Biotin
BAF2085
Phycoerythrin
IC2085P
Unconjugated
AF2085
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF2085
Carrier Free
AF2085
Carrier Free
IC2085P
Carrier Free
IC2085A
Carrier Free
IC2085G
Carrier Free
AF2085-SP
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Brachyury Antibody [NL557] Summary

Immunogen
E. coli‑derived, recombinant human Brachyury
Ser2-Glu202
Accession # O15178
Specificity
Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
T
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 1:10 dilution

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide. See Certificate of Analysis for details.
*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brachyury Antibody [NL557]

  • brachyury protein
  • Brachyury
  • MGC104817
  • Protein T
  • SAVA
  • T brachyury (mouse) homolog
  • T brachyury homolog
  • T Brachyury Transcription Factor
  • T, brachyury homolog (mouse)
  • T-Box Transcription Factor T
  • TBXT
  • TF
  • TFT

Background

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol T
Uniprot