BMP-5 Antibody (149107) Summary
Immunogen |
Mouse myeloma cell line NS0-derived recombinant human BMP‑5 Asn313-His454 Accession # P22003 |
Specificity |
Detects human BMP-5 in direct ELISAs and Western blots. In direct ELISAs, 50% cross-reactivity with recombinant human (rh) BMP-6 is observed and no cross-reactivity with rhBMP-2, -3, -4, or -7 is observed. |
Source |
N/A |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
BMP5 |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Endotoxin Note |
<0.10 EU per 1 μg of the antibody by the LAL method. |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Neutralization 2-10 ug/mL
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for BMP-5 Antibody (149107)
Background
Bone
Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and
functionally related BMPs which are members of the transforming growth
factor beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 454 amino
acid (aa) precursor protein that is cleaved at the dibasic cleavage site
(RxxR) to release the 20 kDa C-terminal mature protein (2). Mature
BMP-5 contains seven conserved cysteine residues involved in formation
of the cysteine knot and the single interchain disulfide bond.
Biologically active BMP-5 is a disulfide-linked homodimer of the
C-terminal mature protein. Mature human BMP-5 shares 96% aa sequence
identity with mouse and rat BMP-5. Cellular responses to BMP-5 are
mediated by the formation of hetero-oligomeric complexes of type I and
type II serine/threonine kinase receptors (1). BMP-5 is expressed by
chondrocytes in proliferating and hypertrophic zones of bone growth
plates (3). It contributes to limb development by promoting
proliferation and differentiation of chondrocytes as well as apoptosis
of undifferentiated mesoderm (3, 4). Genetic defects in BMP-5 which
cause C-terminal truncation or loss of the proteolytic cleavage site
result in multiple skeletal abnormalities, including the short ear
phenotype in mice (5, 6). BMP-5 is also expressed by ovarian granulosa
cells where it functions as an autocrine factor to promote GC
proliferation and inhibit their production of progesterone (7). In the
nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic
neuronal differentiation (8, 9). It is upregulated in oral squamous
carcinoma cells and induces the apoptosis of some myeloma cell lines
(10, 11).
- Chen, D. et al. (2004) Growth Factors 22:233.
- Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
- Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.
- Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.
- King, J.A. et al. (1994) Dev. Biol. 166:112.
- Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.
- Pierre, A. et al. (2005) Biol. Reprod. 73:1102.
- Beck, H.N. et al. (2001) BMC Neurosci. 2:12.
- Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.
- Jin, Y. et al. (2001) Oral Oncol. 37:225.
- Ro, T.B. et al. (2004) Oncogene 23:3024.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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