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Recombinant Human BMP-4 Protein

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SDS-Page: Human BMP-4 Protein [NBP2-34931]
SDS-Page: Human BMP-4 Protein [NBP2-34931]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PAGE
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-34931-100ug
Carrier-Free
NBP2-34931-500ug
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Recombinant Human BMP-4 Protein Summary

Description
A monomeric, non-glycosylated polypeptide chain containing 117 amino acids corresponding to BMP-4 Source: E. coli

Uniprot ID: P12644

Amino Acid Sequence: MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
BMP4
Purity
>95%, by SDS-PAGE
Endotoxin Note
Less than 1 EU/ug of BMP-4 as determined by LAL method.

Applications/Dilutions

Dilutions
  • ELISA
  • SDS-Page
  • Western Blot
Application Notes
1. Molecular standard (Western, ELISA) in studying secreted BMP-4;
2. Preparing antibodies for BMP-4 monomer;
3. Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.
Theoretical MW
13.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 50 mM Na2CO3, 5 mM DTT, 0.02% Tween 20, pH 11.0.
Preservative
5% Trehalose
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water to a concentration of 0.1-1.0mg/mL

Alternate Names for Recombinant Human BMP-4 Protein

  • BMP2B
  • BMP-2B
  • BMP2B1
  • BMP2BMCOPS6
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • MCOPS6
  • OFC11
  • ZYME

Background

BMP-4 plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Mature BMP-4 forms homodimers and heterodimers with BMP-7. BMP-4/BMP-7 heterodimers show greater potency at osteogenic differentiation compared to BMP-4 homodimers. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human
BMP-4 Protein
NBP2-34931
Recombinant Human BMP-4 Protein
Species: Hu
Applications: WB, ELISA, PAGE

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Bioinformatics

Gene Symbol BMP4
Uniprot