BMP-4 Antibody (1050731) [Unconjugated] Summary
Immunogen |
E. coli-derived human BMP-4 protein Lys303-Arg408 Accession # P12644 |
Specificity |
Detects human BMP-4 in direct ELISAs |
Source |
N/A |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry 8 ug/mL
|
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for BMP-4 Antibody (1050731) [Unconjugated]
Background
BMP-4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1, 5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP-4/BMP-7 disulfidelinked heterodimer (7, 9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13-15).
- Zhang, P. et al. (2008) Blood 111:1933.
- Gambaro, K. et al. 2006, Cell Death Differ. 13:1075.
- Simic, P. and Vukicevic, S. (2005) Cytokine Growth Factor Rev. 16:299.
- Sadlon, T.J. et al. 2004, Stem Cells 22:457.
- Frank, D.B. et al. 2005, Circ. Res. 97:496.
- Wozney, J. et al. (1988) Science 242:1528.
- Cui, Y. et al. (1998) EMBO J. 17:4735.
- Cui, Y. et al. (2001) Genes Dev. 15:2797.
- Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
- Nishimatsu, S. and G.H. Thomsen, (1998) Mech. Dev. 74:75.
- Chen, D. et al. (2004) Growth Factors 22:233.
- Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
- Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
- Jones, C.M. and J.C. Smith, (1998) Dev. Biol. 194:12.
- Takada, T. et al. (2003) J. Biol. Chem. 278:43229.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: BA
Species: Mu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: IHC, Simple Western, WB
Species: Hu, Mu
Applications: ELISA, ICC/IF, WB
Species: Hu, Mu
Applications: BA
Species: Hu, Mu
Applications: IHC, WB
Species: Hu
Applications: BA
Species: Hu
Applications: ELISA
Species: Mu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: Block, IHC
Species: Hu
Applications: CyTOF-ready, Flow, IHC
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
Species: Hu
Applications: ICC/IF
Publications for BMP-4 Antibody (MAB7572) (0)
There are no publications for BMP-4 Antibody (MAB7572).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for BMP-4 Antibody (MAB7572) (0)
There are no reviews for BMP-4 Antibody (MAB7572).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
FAQs for BMP-4 Antibody (MAB7572) (0)
Secondary Antibodies
| |
Isotype Controls
|
Additional BMP-4 Products
Blogs on BMP-4