![Western Blot: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, LNCaP, RKO, Hep-G2, U2OS, A-549, Jurkat, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0016.jpg "Western Blot: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, LNCaP, RKO, Hep-G2, U2OS, A-549, Jurkat, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3.")
| Reactivity | Hu, Mu, Bv, Ch, Fe, PmSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 276 and 326 of human B lymphoma Mo-MLV insertion region 1 homolog using the numbering given in entry NP_005171.4 (GeneID 648). |
| Predicted Species | Chicken (100%), Primate (100%), Feline (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BMI1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Other | 07/29/2009 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for BMI-1 Antibody (NB100-87026)Find related products by research area.
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![Immunohistochemistry-Paraffin: BMI-1 Antibody [NB100-87026] - Human pancreatic islet cell carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NB100-87026-img0010.jpg "Immunohistochemistry-Paraffin: BMI-1 Antibody [NB100-87026] - Human pancreatic islet cell carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).")
![Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 on HeLa whole cell lysate using NB100-87026. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0011.jpg "Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 on HeLa whole cell lysate using NB100-87026. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody.")
![Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0013.jpg "Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: BMI-1 Antibody [NB100-87026] - Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-RPS3.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NB100-87026-img0014.jpg "Western Blot: BMI-1 Antibody [NB100-87026] - Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-RPS3.")
![Immunoprecipitation: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for IP at 6 ug/mg lysate. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NB100-87026-img0015.jpg "Immunoprecipitation: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for IP at 6 ug/mg lysate. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")