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beta-III Tubulin Antibody (TuJ-1) [Biotin] - Neuron-Specific

 (7 Publications)
 (1 Review)
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beta -III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using Neuron-specific beta -III Tubulin Biotinylated Monoclonal Antibody (Catalog # BAM1195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta -III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using Neuron-specific beta -III Tubulin Biotinylated Monoclonal Antibody (Catalog # BAM1195) at 10 µg/mL for 3 hours at ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, ICC/IF
Clone
TuJ-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
IC1195A
NL493
NL1195G
NL557
NL1195R
NL637
NL1195V
PerCP (Peridinin-chlorophyll Protein Complex)
IC1195C
Unconjugated
MAB1195
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1195
Carrier Free
IC1195C
Carrier Free
MAB1195
Carrier Free
IC1195A
Carrier Free
NL1195R
Carrier Free
NL1195G
Carrier Free
NL1195V
Carrier Free
MAB1195-SP
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beta-III Tubulin Antibody (TuJ-1) [Biotin] - Neuron-Specific Summary

Immunogen
Rat brain-derived microtubules
Specificity
Detects mammalian and chicken neuron-specific  beta -III tubulin but not other beta -tubulin isotypes.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TUBB3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Readout System
Reviewed Applications
Read 1 Review rated 5
using
BAM1195 in the following applications:
Publications
Read Publications using
BAM1195 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-III Tubulin Antibody (TuJ-1) [Biotin] - Neuron-Specific

  • Beta3-tubulin
  • beta-4
  • betaIII Tubulin
  • beta-III Tubulin
  • beta-Tubulin III
  • Class III beta-tubulin
  • TUBB3
  • TUBB4 CFEOM3A
  • Tubulin Beta 3
  • tubulin beta-3 chain
  • Tubulin beta-4 chain
  • Tubulin beta-4
  • Tubulin beta-III
  • tubulin, beta 3
  • TUJ1 antigen
  • TUJ-1 antigen

Background

Class III beta tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of class III beta -tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium (1, 2).

  1. Caccamo, D. et al. (1989) Lab. Invest. 60:390.
  2. Alexander, J. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4685.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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beta-III Tubulin
Antibody (TuJ-1) [Bio ...
BAM1195
beta-III Tubulin Antibody (TuJ-1) [Biotin] - Neuron-Specific
Species: All-Multi
Applications: WB, ICC/IF

Publications for beta-III Tubulin Antibody (BAM1195)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-Fr, IHC-P.


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(1)
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Showing Publications 1 - 7 of 7.
Publications using BAM1195 Applications Species
Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life Mucosal Immunol, 2016-02-10;9(6):1466-1476. 2016-02-10 [PMID: 26860818] (IHC-P, Mouse) IHC-P Mouse
Aven , Linh, Paez-Cortez , Jesus, Achey , Rebecca, Krishnan , Ramaswam, Ram-Mohan , Sumati, Cruikshank , William, Fine , Alan, Ai , Xingbin An NT4/TrkB-dependent increase in innervation links early-life allergen exposure to persistent airway hyperreactivity. FASEB J, 2013-11-12;28(2):897-907. 2013-11-12 [PMID: 24221086] (IHC-Fr, Mouse) IHC-Fr Mouse
Randee E. Young, Mary-Kayt Jones, Elizabeth A. Hines, Rongbo Li, Yongfeng Luo, Wei Shi et al. Smooth Muscle Differentiation Is Essential for Airway Size, Tracheal Cartilage Segmentation, but Dispensable for Epithelial Branching Developmental Cell 4/1/2020 [PMID: 32142630]
Claudia Prahst, Parham Ashrafzadeh, Thomas Mead, Ana Figueiredo, Karen Chang, Douglas Richardson et al. Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy eLife 2/19/2020 [PMID: 32073398]
Aleksander D Szymaniak, Rongjuan Mi, Shannon E McCarthy, Adam C Gower, Taylor L Reynolds, Michael Mingueneau et al. The Hippo pathway effector YAP is an essential regulator of ductal progenitor patterning in the mouse submandibular gland eLife 5/11/2017 [PMID: 28492365]
Kelsi Radzikinas, Linh Aven, Zhihua Jiang, Thanh Tran, Jesus Paez-Cortez, Karthik Boppidi et al. A Shh/miR-206/BDNF Cascade Coordinates Innervation and Formation of Airway Smooth Muscle The Journal of Neuroscience 10/26/2011 [PMID: 22031887]

Review for beta-III Tubulin Antibody (BAM1195) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using BAM1195:
Filter by Applications
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(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 IHC-Fr Mouse 01/15/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID: 22031887
SpeciesMouse

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 Verified Customer
01/15/2015
Application: IHC-Fr
Species: Mouse
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Bioinformatics

Gene Symbol TUBB3