![Western Blot: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of Tubulin Beta 3 mouse mAb against (1) HepG2, (2) A549, and (3) Hela cell lysates.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2E9-Western-Blot-NBP1-42568-img0001.jpg "Western Blot: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of Tubulin Beta 3 mouse mAb against (1) HepG2, (2) A549, and (3) Hela cell lysates.")
| Reactivity | Hu, Rt, Mu, Bv, PmSpecies Glossary |
| Applications | WB, Simple Western, ELISA, Flow, ICC/IF, IHC |
| Clone | 2E9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Full length recombinant human Tubulin Beta 3 expressed in E. coli. [UniProt# Q13509]. |
| Marker | Neuronal Marker |
| Predicted Species | Mouse (99%), Bovine (99%), Monkey (100%). Backed by our 100% Guarantee. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TUBB3 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Ascites |
| Preservative | 0.03% Sodium Azide |
| Purity | Unpurified |
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| Images | Ratings | Applications | Species | Date | Details | ||||
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-(01-ml)_NBP1-42568_8406.bmp)
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reviewed by:
Bryan Tinsley |
ICC | Human | 06/20/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Confocal analysis of SHSY5Y cells using Tubulin Beta 3 antibody (NBP1-42568, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2E9-Immunocytochemistry-Immunofluorescence-NBP1-42568-img0004.jpg "Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Confocal analysis of SHSY5Y cells using Tubulin Beta 3 antibody (NBP1-42568, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).")
![Flow Cytometry: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of A549 cells using anti-Tubulin Beta 3 mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2E9-Flow-Cytometry-NBP1-42568-img0003.jpg "Flow Cytometry: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of A549 cells using anti-Tubulin Beta 3 mAb (green) and negative control (purple).")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of PANC-1 cells using Tubulin Beta 3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2E9-Immunocytochemistry-Immunofluorescence-NBP1-42568-img0002.jpg "Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Analysis of PANC-1 cells using Tubulin Beta 3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Simple Western: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Lane view shows a specific band for Tubulin Beta 3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2E9-Simple-Western-NBP1-42568-img0005.jpg "Simple Western: beta-III Tubulin Antibody (2E9) [NBP1-42568] - Lane view shows a specific band for Tubulin Beta 3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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![SDS-Page: HepG2 Whole Cell Lysate [NBP1-42569] - Ponceau S staining of HepG2 whole cell lysate using NBP1-42569.](https://images.novusbio.com/images/HepG2-Whole-Cell-Lysate-SDS-Page-NBP1-42569-img0001.jpg "SDS-Page: HepG2 Whole Cell Lysate [NBP1-42569] - Ponceau S staining of HepG2 whole cell lysate using NBP1-42569.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
-(01-ml)_NBP1-42568_8406.bmp)