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Beta Ig-h3/TGFBI Antibody

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Western Blot: Beta Ig-h3/TGFBI Antibody [NBP1-47215] - Staining of Human Kidney (A), Colorectal cancer (B) cell lysate with antibody at 0.3 ug/mL, Human Prostate (C) and Rat Kidney (D) cell lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot: Beta Ig-h3/TGFBI Antibody [NBP1-47215] - Staining of Human Kidney (A), Colorectal cancer (B) cell lysate with antibody at 0.3 ug/mL, Human Prostate (C) and Rat Kidney (D) cell lysate with antibody at 0.1 ...read more
Immunocytochemistry/ Immunofluorescence: Beta Ig-h3/TGFBI Antibody [NBP1-47215] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) ...read more
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-47215] - Staining of paraffin embedded human kidney. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Rt, Mu, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Beta Ig-h3/TGFBI Antibody Summary

Immunogen
Peptide with sequence C-QLYTDRTEKLRPE corresponding to internal region according to NP_000349.1.
Epitope
Internal region
Predicted Species
Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFBI
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 4 - 6 ug/mL
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 4 - 6 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/mL
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:35063020)
Publications
Read Publications using
NBP1-47215 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Beta Ig-h3/TGFBI Antibody

  • beta IGH3
  • beta IG-H3
  • BIGH3EBMD
  • CDB1
  • CDG2
  • CDGG1transforming growth factor, beta-induced, 68kD
  • CSD
  • CSD1
  • CSD2
  • CSD3
  • Kerato-epithelin
  • LCD1
  • RGD-CAP
  • RGD-containing collagen-associated protein
  • TGFBI
  • TGFBIP
  • transforming growth factor, beta-induced, 68kDa
  • transforming growth factor-beta-induced protein ig-h3

Background

TGFBI, also known as BIGH3 and betaig-h3, is an extracellular matrix protein induced by transforming growth factor (TGF)-beta 1. TGFBI protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of TGFBI were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain). Clone k1H12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TGFBI protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Beta Ig-h3/TGFBI
Antibody
NBP1-47215
Beta Ig-h3/TGFBI Antibody
Species: Hu, Rt, Mu, Ca
Applications: WB, ELISA, ICC/IF, IHC

Publications for Beta Ig-h3/TGFBI Antibody (NBP1-47215)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IHC-Fr.


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Bioinformatics

Gene Symbol TGFBI
Entrez
Uniprot