![Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Western-Blot-NBP2-80596-img0004.jpg "Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, Av-Du, Ch, PmSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | 12F7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant chicken beta Catenin fused to maltose binding protein. [UniProt# O42486] |
| Localization | Nucleus, Cytoplasm, Cytoskeleton, Adherens junction, Centrosome, Spindle pole. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CTNNB1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | This beta Catenin (12F7) antibody is useful for IHC-P sections, ICC/IF, IP and WB, where a band can be seen at approx. 92 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
| Theoretical MW | 92 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for beta-Catenin Antibody (NBP2-80596)Find related products by research area.
|
|
Read full blog post. |
|
Stemness is responsible for onset and metastasis of colorectal cancer By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C... Read full blog post. |
|
The role of Wnts in neuroinflammation By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s... Read full blog post. |
|
Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy... Read full blog post. |
|
Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
|
CD63: is it pro-metastatic or anti-metastatic? CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,... Read full blog post. |
|
Notch1 - A multifunctional transmembrane receptor Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CTNNB1 |
![Immunocytochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta- Catenin in mouse intestine using DAB with hematoxylin counterstain. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80596-img0009.jpg "Immunocytochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta- Catenin in mouse intestine using DAB with hematoxylin counterstain. Image from the standard format of this antibody.")
![Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta Catenin in mouse intestine using DAB with hematoxylin counterstain. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80596-img0008.jpg "Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta Catenin in mouse intestine using DAB with hematoxylin counterstain. Image from the standard format of this antibody.")
![Flow Cytometry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - An intracellular stain was performed on SK-MEL-28 cells with beta-Catenin Antibody [12F7] NBP1-54467B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80596-img0010.jpg "Flow Cytometry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - An intracellular stain was performed on SK-MEL-28 cells with beta-Catenin Antibody [12F7] NBP1-54467B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals). Image from the standard format of this antibody.")
![Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Activity of Wnt/beta-catenin pathway in the embryonic chick lung. Western blot analysis of active and total beta-catenin in stage b1, b2 and b3 lungs, and stage 24 limb (as positive control). Control loading was performed using beta-tubulin (55 kDa). Total and active beta-catenin correspond to 92 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112388), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Western-Blot-NBP2-80596-img0001.jpg "Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Activity of Wnt/beta-catenin pathway in the embryonic chick lung. Western blot analysis of active and total beta-catenin in stage b1, b2 and b3 lungs, and stage 24 limb (as positive control). Control loading was performed using beta-tubulin (55 kDa). Total and active beta-catenin correspond to 92 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112388), licensed under a CC-BY license. Image from the standard format of this antibody.")
![Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Western-Blot-NBP2-80596-img0002.jpg "Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467. Image from the standard format of this antibody.")
![Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta- Catenin in embryonic lung (lane 1) and embryonic limb (lane 2) lysates using anti-beta- Catenin antibody. Each lane was loaded with 5ug of protein sample. Image from verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Western-Blot-NBP2-80596-img0003.jpg "Western Blot: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Analysis of beta- Catenin in embryonic lung (lane 1) and embryonic limb (lane 2) lysates using anti-beta- Catenin antibody. Each lane was loaded with 5ug of protein sample. Image from verified customer review. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80596-img0005.jpg "Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue). Image from the standard format of this antibody.")
![Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Beta-catening (green) was detected in human skin (psoriasis) using beta-catenin-FITC antibody (1:40) in PBS for 1 hour. Nuclei were stained with Dapi (blue). Image from verified customer review. Image using the FITC format of this antibody. Image from the](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80596-img0006.jpg "Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Beta-catening (green) was detected in human skin (psoriasis) using beta-catenin-FITC antibody (1:40) in PBS for 1 hour. Nuclei were stained with Dapi (blue). Image from verified customer review. Image using the FITC format of this antibody. Image from the")
![Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - IF beta-Catenin staining of mouse brain tissue. This image submitted by a verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80596-img0007.jpg "Immunohistochemistry: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - IF beta-Catenin staining of mouse brain tissue. This image submitted by a verified customer review. Image from the standard format of this antibody.")
![Simple Western: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Simple Western lane view shows a specific band for Beta- Catenin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Azide-and-BSA-Free-Simple-Western-NBP2-80596-img0011.jpg "Simple Western: beta-Catenin Antibody (12F7) - Azide and BSA Free [NBP2-80596] - Simple Western lane view shows a specific band for Beta- Catenin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")