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beta-Catenin Antibodies

We offer beta-Catenin Antibodies for use in common research applications: Chromatin Immunoprecipitation (ChIP), Dot Blot, ELISA, Flow (Intracellular), Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each beta-Catenin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our beta-Catenin Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Fish, Hamster, Human, Monkey, Mouse, Opossum, Porcine, Primate, Rat, Xenopus, Zebrafish. Use the list below to choose the beta-Catenin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our beta-Catenin polyclonal antibodies and browse our beta-Catenin monoclonal antibody catalog.

Alternate Names for beta-Catenin Antibodies

anti-beta-Catenin antibody, anti-CTNNB1 antibody, anti-beta 1 (88kD) antibody, anti-catenin (cadherin-associated protein), beta 1, 88kDa antibody, anti-catenin beta-1 antibody, anti-CTNNB antibody, anti-DKFZp686D02253 antibody, anti-FLJ25606 antibody, anti-FLJ37923 antibody

47 Results for "beta-catenin" in Primary Antibodies

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AF1329

beta-Catenin Antibody [Unconjugated]

(2 Reviews)

(33 Publications)

100 μg

AF1329

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human beta -catenin (1 ng) was included. A specific band for beta-Catenin was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HeLa human cervical epithelial carcinoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. beta ‑Catenin/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) or control antibody (Catalog # AB‑108‑C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The SU(Z)12 promoter was detected by standard PCR.

beta -Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

beta -Catenin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

beta -Catenin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 15 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in collecting tubules in the medulla. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1329, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Catenin at approximately 94-97 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF1329

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1329-SP

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NBP1-54467

beta-Catenin Antibody (12F7) - BSA Free

(4 Reviews)

(22 Publications)

0.1 ml

NBP1-54467

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).

Immunohistochemistry: beta-Catenin Antibody (12F7) [NBP1-54467] - Beta-catening (green) was detected in human skin (psoriasis) using beta-catenin-FITC antibody (1:40) in PBS for 1 hour. Nuclei were stained with Dapi (blue). Image from verified customer review. Image using the FITC format of this antibody.

Flow Cytometry: beta-Catenin Antibody (12F7) [NBP1-54467] - An intracellular stain was performed on SK-MEL-28 cells with beta-Catenin Antibody [12F7] NBP1-54467B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467.

Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta- Catenin in embryonic lung (lane 1) and embryonic limb (lane 2) lysates using anti-beta- Catenin antibody. Each lane was loaded with 5ug of protein sample. Image from verified customer review.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta Catenin in mouse intestine using DAB with hematoxylin counterstain.

Immunohistochemistry: beta-Catenin Antibody (12F7) [NBP1-54467] - IF beta-Catenin staining of mouse brain tissue. This image submitted by a verified customer review.

Flow (Intracellular): beta-Catenin Antibody (12F7) [NBP1-54467] - An intracellular stain was performed on HeLa cells with beta-Catenin Antibody (12F7) NBP1-54467AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Simple Western: beta-Catenin Antibody (12F7) [NBP1-54467] - Simple Western lane view shows a specific band for Beta- Catenin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Activity of Wnt/ beta -catenin pathway in the embryonic chick lung.(A) Western blot analysis of active and total beta -catenin in stage b1, b2 and b3 lungs, and stage 24 limb (as positive control). Control loading was performed using beta -tubulin (55 kDa). Total and active beta -catenin correspond to 92 kDa. (B) Semi-quantitative analysis for active and total beta -catenin. Results are presented as arbitrary units normalized for beta -tubulin. p<0.05: * vs limb.

Reactivity:

Hu, Mu, Rt, Av-Du, Ch, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Av-Du, Ch, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-54467AF350
Alexa Fluor 405	NBP1-54467AF405
Alexa Fluor 488	NBP1-54467AF488
Alexa Fluor 532	NBP1-54467AF532
Alexa Fluor 594	NBP1-54467AF594
Alexa Fluor 647	NBP1-54467AF647
Alexa Fluor 700	NBP1-54467AF700
Alexa Fluor 750	NBP1-54467AF750
Biotin	NBP1-54467B
DyLight 350	NBP1-54467UV
DyLight 405	NBP1-54467V
DyLight 488	NBP1-54467G
DyLight 550	NBP1-54467R
DyLight 594	NBP1-54467DL594
DyLight 650	NBP1-54467C
DyLight 680	NBP1-54467FR
DyLight 755	NBP1-54467IR
FITC	NBP1-54467F
HRP	NBP1-54467H
Janelia Fluor 549	NBP1-54467JF549
Janelia Fluor 646	NBP1-54467JF646
mFluor Violet 450 SE	NBP1-54467MFV450
mFluor Violet 500 SE	NBP1-54467MFV500
mFluor Violet 610 SE	NBP1-54467MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-54467SS
Azide and BSA Free	NBP2-80596

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NBP1-32239

beta-Catenin Antibody

(1 Review)

(8 Publications)

100 ul

NBP1-32239

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - WNT10A/beta-catenin signaling is required for region-specific differentiation. Epithelial deletion of beta-catenin (g-h

Flow Cytometry: beta-Catenin Antibody [NBP1-32239] - HeLa cell.Black: Unlabelled sample was used as a control. Red: beta Catenin antibody [N1N2-2], N-term dilution: 1:50.Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Chromatin Immunoprecipitation (ChIP): beta-Catenin Antibody [NBP1-32239] - Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ug of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.

Western Blot: beta-Catenin Antibody [NBP1-32239] - Western blot for beta-catenin in HEK 293 cells treated with LiCl (10mM) for 1 hr. Image from verified customer review.

Western Blot: beta-Catenin Antibody [NBP1-32239] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000

Western Blot: beta-Catenin Antibody [NBP1-32239] - A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGEbeta Catenin antibody [N1N2-2], N-term dilution: 1:1000

<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term.

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse colon. Green: beta Catenin antibody [diluted at 1:500. Red: alpha Tubulin antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse urinary bladder diluted at 1:500.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse skin dilution: 1:500.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse colon dilution: 1:500.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human esophagus. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human cervix. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat colon. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse intestine. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - P-cadherin is co-localized with other junctional proteins at the RPE cell border in mice.Immunofluorescence of mouse RPE flat-mounts. Double staining: P-cadherin (red; A, E, I) & either ZO-1 (green; B), beta -catenin (green; F), or F-actin (green; J), with nuclear stain by DAPI (blue; C, G, K). Merged images (D, H, L) show the co-localization of P-cadherin with ZO-1 (tight junction), beta -catenin (adherens junction), & F-actin (adherens junction) at the cell-cell border. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29338041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - Oxidative stress-induced dissociation of adherens junctions results in nuclear translocation of beta -catenin & an increase of EMT-related factors in mouse RPE.(A) Immunofluorescence of mouse RPE flat-mounts. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & the localization of beta -catenin (green) & P-cadherin (red) was analyzed along with nuclear stain by DAPI (blue) on Days 0 (a-c), 1 (d-f), 3 (g-i) & 7 (j-l). Double staining: beta -catenin (a, d, g, j), P-cadherin (b, e, h, k), & merged images with DAPI (c, f, i, l). The localization of beta -catenin & P-cadherin at the cell-cell border was significantly disrupted, & instead prominently detected on/in the nucleus on Day 3. (B) Immunofluorescence of mouse retinal sections with a focus on the RPE nuclei. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & the localization of beta -catenin (green) & P-cadherin (red) was analyzed along with nuclear stain by DAPI (blue) on Days 0 (m-o) & 3 (two representative nuclei; p-r & s-u). Double staining: beta -catenin (m, p, s), P-cadherin (n, q, t), & merged images with DAPI (o, r, u). On Day 3, beta -catenin was detected in the nuclei of mouse RPE. (C) Western blot analyses of mouse RPE proteins. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & RPE protein lysates were prepared on Days 0, 1, 3, & 7. The protein levels were analyzed using Western blotting with antibodies against P-cadherin, beta -catenin, SNAI1 (Snail), vimentin, & control beta -actin. The protein levels of beta -catenin & SNAI1 increased similarly on Day 1 following oxidative stress. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29338041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta-Catenin Antibody [NBP1-32239] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:100.

Western Blot: beta-Catenin Antibody [NBP1-32239] - Various tissue extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - Immunohistochemical analysis of agarose-embedded zebrafish embryo, using beta-Catenin antibody [N1N2-2], N-term NBP1-32239) at 1:100. dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects beta-Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.beta-Catenin stained by beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2] detects beta-Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta-Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500.Red: alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:1000.

Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) at 1:300 dilution.

<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (9 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Ca, Fe, Rb, Ze, Po, Bv, Ch, RM, Sh, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Fe, Rb, Ze, Po, Bv, Ch, RM, Sh, Xp

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB13291

beta-Catenin Antibody (196618) [Unconjugated]

(8 Publications)

50 μg

MAB13291

beta -Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using 10 µg/mL Mouse Anti-Human beta -Catenin Monoclonal Antibody (Catalog # MAB13291) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

beta -Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human beta -Catenin Monoclonal Antibody (Catalog # MAB13291) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB13291-100
Carrier Free	MAB13291-SP

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MAB1329

beta-Catenin Antibody (196621) [Unconjugated]

(5 Publications)

50 μg

MAB1329

beta-Catenin was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human beta-Catenin Monoclonal Antibody (Catalog # MAB1329) at 15 µg/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and cell membrane. Protocol available in COMET™ Panel Builder.

Western blot shows lysates of Huh-7 human hepatoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat beta -Catenin Monoclonal Antibody (Catalog # MAB1329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta -Catenin at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

beta -Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse/Rat beta -Catenin Monoclonal Antibody (Catalog # MAB1329) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1329-200
Carrier Free	MAB1329-SP

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NBP1-89989

beta-Catenin Antibody

(3 Publications)

0.1 ml

NBP1-89989

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-89989] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.

Western Blot: beta-Catenin Antibody [NBP1-89989] - Analysis in human cell line SW480.

Western Blot: beta-Catenin Antibody [NBP1-89989] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human cervix, uterine shows moderate membranous positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human duodenum shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human prostate shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human skeletal muscle shows weak positivity in myocytes.

Reactivity:

Hu, Mu, Rt, Fi

Species Glossary

Reactivity: Hu, Mu, Rt, Fi

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-03615

beta-Catenin Antibody (OTI12H7)

0.1 ml

NBP2-03615

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.

Flow Cytometry: beta-Catenin Antibody (12H7) [NBP2-03615] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-beta Catenin antibody, and then analyzed by flow cytometry.

Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta Catenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-beta Catenin.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.

Flow Cytometry: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of Hela cells, using anti-beta Catenin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Reactivity:

Hu, Mu, Rt, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70509AF350
Alexa Fluor 405	NBP2-70509AF405
Alexa Fluor 488	NBP2-70509AF488
Alexa Fluor 532	NBP2-70509AF532
Alexa Fluor 594	NBP2-70509AF594
Alexa Fluor 647	NBP2-70509AF647
Alexa Fluor 700	NBP2-70509AF700
Alexa Fluor 750	NBP2-70509AF750
Allophycocyanin	NBP2-70509APC
Biotin	NBP2-70509B
CoraFluor 1	NBP2-70509CL1
DyLight 350	NBP2-70509UV
DyLight 405	NBP2-70509V
DyLight 488	NBP2-70509G
DyLight 550	NBP2-70509R
DyLight 594	NBP2-70509DL594
DyLight 650	NBP2-70509C
DyLight 680	NBP2-70509FR
DyLight 755	NBP2-70509IR
FITC	NBP2-70509F
HRP	NBP2-70509H
Janelia Fluor 525	NBP2-70509JF525
Janelia Fluor 549	NBP2-70509JF549
Janelia Fluor 646	NBP2-70509JF646
Janelia Fluor 669	NBP2-70509JF669
PE	NBP2-70509PE
PerCP	NBP2-70509PCP
mFluor Violet 450 SE	NBP2-70509MFV450
mFluor Violet 500 SE	NBP2-70509MFV500
mFluor Violet 610 SE	NBP2-70509MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70509

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NBP2-70509

beta-Catenin Antibody (OTI12H7) - Azide and BSA Free

100 μg

NBP2-70509

Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.

Flow Cytometry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-beta Catenin antibody, and then analyzed by flow cytometry.

Western Blot: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Analysis of extracts (35ug) from 9 different cell lines by using anti-beta Catenin monoclonal antibody.

Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Staining of paraffin-embedded Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.

Flow Cytometry: beta-Catenin Antibody (OTI12H7) - Azide and BSA Free [NBP2-70509] - Analysis of Hela cells, using anti-beta Catenin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Reactivity:

Hu, Mu, Rt, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70509AF350
Alexa Fluor 405	NBP2-70509AF405
Alexa Fluor 488	NBP2-70509AF488
Alexa Fluor 532	NBP2-70509AF532
Alexa Fluor 594	NBP2-70509AF594
Alexa Fluor 647	NBP2-70509AF647
Alexa Fluor 700	NBP2-70509AF700
Alexa Fluor 750	NBP2-70509AF750
Allophycocyanin	NBP2-70509APC
Biotin	NBP2-70509B
CoraFluor 1	NBP2-70509CL1
DyLight 350	NBP2-70509UV
DyLight 405	NBP2-70509V
DyLight 488	NBP2-70509G
DyLight 550	NBP2-70509R
DyLight 594	NBP2-70509DL594
DyLight 650	NBP2-70509C
DyLight 680	NBP2-70509FR
DyLight 755	NBP2-70509IR
FITC	NBP2-70509F
HRP	NBP2-70509H
Janelia Fluor 525	NBP2-70509JF525
Janelia Fluor 549	NBP2-70509JF549
Janelia Fluor 585	NBP2-70509JF585
Janelia Fluor 635	NBP2-70509JF635
Janelia Fluor 646	NBP2-70509JF646
Janelia Fluor 669	NBP2-70509JF669
PE	NBP2-70509PE
PerCP	NBP2-70509PCP
mFluor Violet 450 SE	NBP2-70509MFV450
mFluor Violet 500 SE	NBP2-70509MFV500
mFluor Violet 610 SE	NBP2-70509MFV610

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NBP2-53366

beta-Catenin Antibody (CTNNB1/1509)

(1 Review)

100 μg

NBP2-53366

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded human Pancreas stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1509) [NBP2-53366] - Formalin-fixed, paraffin-embedded Mouse Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1509).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54540AF350
Alexa Fluor 405	NBP2-54540AF405
Alexa Fluor 488	NBP2-54540AF488
Alexa Fluor 532	NBP2-54540AF532
Alexa Fluor 594	NBP2-54540AF594
Alexa Fluor 647	NBP2-54540AF647
Alexa Fluor 700	NBP2-54540AF700
Alexa Fluor 750	NBP2-54540AF750
Biotin	NBP2-54540B
CoraFluor 1	NBP2-54540CL1
DyLight 350	NBP2-54540UV
DyLight 405	NBP2-54540V
DyLight 488	NBP2-54540G
DyLight 550	NBP2-54540R
DyLight 594	NBP2-54540DL594
DyLight 650	NBP2-54540C
DyLight 680	NBP2-54540FR
DyLight 755	NBP2-54540IR
FITC	NBP2-54540F
HRP	NBP2-54540H
Janelia Fluor 525	NBP2-54540JF525
Janelia Fluor 549	NBP2-54540JF549
Janelia Fluor 646	NBP2-54540JF646
Janelia Fluor 669	NBP2-54540JF669

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54540-100ug

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NBP2-46400

beta-Catenin Antibody (OTI9C1)

0.1 ml

NBP2-46400

Western Blot: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.

Immunohistochemistry: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunofluorescence: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY beta-Catenin.

Immunofluorescence: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of HeLa cells.

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, ,

Applications: , ,

Host:

Mouse monoclonal

Host: Mouse monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70508AF350
Alexa Fluor 405	NBP2-70508AF405
Alexa Fluor 488	NBP2-70508AF488
Alexa Fluor 532	NBP2-70508AF532
Alexa Fluor 594	NBP2-70508AF594
Alexa Fluor 647	NBP2-70508AF647
Alexa Fluor 700	NBP2-70508AF700
Alexa Fluor 750	NBP2-70508AF750
Allophycocyanin	NBP2-70508APC
Biotin	NBP2-70508B
CoraFluor 1	NBP2-70508CL1
DyLight 350	NBP2-70508UV
DyLight 405	NBP2-70508V
DyLight 488	NBP2-70508G
DyLight 550	NBP2-70508R
DyLight 594	NBP2-70508DL594
DyLight 650	NBP2-70508C
DyLight 680	NBP2-70508FR
DyLight 755	NBP2-70508IR
FITC	NBP2-70508F
HRP	NBP2-70508H
Janelia Fluor 525	NBP2-70508JF525
Janelia Fluor 549	NBP2-70508JF549
Janelia Fluor 646	NBP2-70508JF646
Janelia Fluor 669	NBP2-70508JF669
PE	NBP2-70508PE
PerCP	NBP2-70508PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70508

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NBP3-15842

beta-Catenin Antibody (6A3P2)

100 ul

NBP3-15842

Immunohistochemistry-Paraffin: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunohistochemistry of paraffin-embedded Human pancreatic ductal carcinoma using [KO Validated] beta-Catenin Rabbit mAb (NBP3-15842) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunohistochemistry of paraffin-embedded Human cervical squamous cell carcinoma using [KO Validated] beta-Catenin Rabbit mAb (NBP3-15842) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunohistochemistry of paraffin-embedded Human Invasive ductal carcinoma of breast using [KO Validated] beta-Catenin Rabbit mAb (NBP3-15842) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using [KO Validated] beta-Catenin Rabbit mAb (NBP3-15842) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

<b>Genetic Strategies Validation. </b>Knockout Validated: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts from normal (control) and beta-Catenin knockout (KO) 293T cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunoprecipitation: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug beta-Catenin antibody (NBP3-15842). Western blot was performed from the immunoprecipitate using beta-Catenin (NBP3-15842) at a dilition of 1:1000.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-67307

beta-Catenin Antibody (SA30-04)

100 ul

NBP2-67307

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Beta-Catenin with Rabbit anti-Beta-Catenin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on different cell lysates using anti- Beta-Catenin antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: HT29 Lane 3: A431

Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on different lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of mouse small intestine tissue using anti-b-Catenin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of mouse colon tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of human colon cancer tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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page of 4

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

beta-Catenin Antibodies

beta-Catenin Antibody [Unconjugated]

beta-Catenin Antibody (12F7) - BSA Free

beta-Catenin Antibody

beta-Catenin Antibody (196618) [Unconjugated]

beta-Catenin Antibody (196621) [Unconjugated]

beta-Catenin Antibody

beta-Catenin Antibody (OTI12H7)

beta-Catenin Antibody (OTI12H7) - Azide and BSA Free

beta-Catenin Antibody (CTNNB1/1509)

beta-Catenin Antibody (OTI9C1)

beta-Catenin Antibody (6A3P2)

beta-Catenin Antibody (SA30-04)