![Western Blot: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis using Beclin 1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-Western-Blot-NBP2-22176-img0004.jpg "Western Blot: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis using Beclin 1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC |
| Clone | 2A4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Purified recombinant fragment of human Beclin 1 expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BECN1 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Ascites |
| Preservative | 0.03% Sodium Azide |
| Purity | Unpurified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | BECN1 |
![Immunohistochemistry-Paraffin: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis of breast cancer tissues (left) and liver cancer tissues (right) using Beclin 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-Immunohistochemistry-Paraffin-NBP2-22176-img0002.jpg "Immunohistochemistry-Paraffin: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis of breast cancer tissues (left) and liver cancer tissues (right) using Beclin 1 mouse mAb with DAB staining.")
![Flow Cytometry: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis of RAJI cells using Beclin 1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-Flow-Cytometry-NBP2-22176-img0001.jpg "Flow Cytometry: Beclin 1 Antibody (2A4) [NBP2-22176] - Analysis of RAJI cells using Beclin 1 mouse mAb (green) and negative control (purple).")
![ELISA: Beclin 1 Antibody (2A4) [NBP2-22176] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Beclin-1-Antibody-2A4-ELISA-NBP2-22176-img0003.jpg "ELISA: Beclin 1 Antibody (2A4) [NBP2-22176] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")