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Bcl-10 Antibody [Unconjugated]

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Bcl-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2889) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bcl-10 at approximately 28 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat ...read more
Bcl-10 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human Bcl-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2889) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2889-SP
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Bcl-10 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Bcl-10
Met1-Leu192
Accession # O95999
Specificity
Detects human Bcl-10 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BCL10
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bcl-10 Antibody [Unconjugated]

  • B-cell CLL/lymphoma 10CIPERCLAPB-cell lymphoma/leukemia 10
  • Bcl10
  • Bcl-10
  • CARD-containing apoptotic signaling protein
  • CARD-containing molecule enhancing NF-kappa-B
  • CARD-containing proapoptotic protein
  • CARD-like apoptotic protein
  • CARMEN
  • caspase-recruiting domain-containing protein
  • cCARMEN
  • c-E10
  • c-E10bcl-10
  • CED-3/ICH-1 prodomain homologous E10-like regulator
  • Cellular homolog of vCARMEN
  • cellular-E10
  • CIPER
  • CLAP
  • hCLAP
  • Mammalian CARD-containing adapter molecule E10
  • mE10
  • mE10CARD containing molecule enhancing NF-kB

Background

B cell CLL/leukemia 10 (Bcl-10), a 233 amino acid, 26.2 kDa protein, is the cellular homolog of the equine herpesvirus-2 gene (E10). Bcl-10 is also known as CARD like apoptotic protein (CLAP), CED-3/ICH-1 prodomain homologous E10-like regulator (CIPER), celluar-E10 (c-E10), and CARD-containing molecule enhancing NF kappa B (CARMEN). Bcl-10 contains a caspase recruitment domain (CARD) and has been shown to induce apoptosis and to activate NF kappa B. Bcl-10 can interact with other CARD domain containing proteins including CARD9, 10, 11, and 14, which function as upstream regulators of NF kappa B signaling. Defects in Bcl‑10 may be involved in the pathogenesis of human malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BCL10
Uniprot