![Western Blot: Bcl-10 Antibody (4F8) [NBP1-51524] - Analysis using BCL10 mouse mAb against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.](http://images.novusbio.com/fullsize/Bcl-10-Antibody-4F8-Western-Blot-NBP1-51524-img0010.jpg "Western Blot: Bcl-10 Antibody (4F8) [NBP1-51524] - Analysis using BCL10 mouse mAb against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 4F8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Bcl-10 expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BCL10 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
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| Theoretical MW | 26 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.03% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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| Publication using NBP1-51524 | Applications | Species |
|---|---|---|
| Bhattacharyya S, Pant N, Dudeja PK, Tobacman JK. Development, evaluation, and application of a highly sensitive microtiter plate ELISA for human Bcl10 protein. J Immunoassay Immunochem 2007-01-01 [PMID: 17613665] (ELISA, Human) | ELISA | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Bcl-10 Antibody (NBP1-51524)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Bcl-10 Antibody (4F8) [NBP1-51524] - Confocal immunofluorescence analysis of Hela cells using BCL10 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Bcl-10-Antibody-4F8-Immunocytochemistry-Immunofluorescence-NBP1-51524-img0011.jpg "Immunocytochemistry/Immunofluorescence: Bcl-10 Antibody (4F8) [NBP1-51524] - Confocal immunofluorescence analysis of Hela cells using BCL10 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.")
![Immunohistochemistry-Paraffin: Bcl-10 Antibody (4F8) [NBP1-51524] - Analysis of human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using BCL10 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Bcl-10-Antibody-4F8-Immunohistochemistry-Paraffin-NBP1-51524-img0008.jpg "Immunohistochemistry-Paraffin: Bcl-10 Antibody (4F8) [NBP1-51524] - Analysis of human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using BCL10 mouse mAb with DAB staining.")
![Flow Cytometry: Bcl-10 Antibody (4F8) [NBP1-51524] - Analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Bcl-10-Antibody-4F8-Flow-Cytometry-NBP1-51524-img0012.jpg "Flow Cytometry: Bcl-10 Antibody (4F8) [NBP1-51524] - Analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")