![Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0006.jpg "Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
| Reactivity | Hu, Mu, Rt, Ha, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 19 amino acids near the amino terminus of human BANF1. The immunogen is located within the first 50 amino acids of BANF1. Amino Acid Sequence: TTSQKHRDFVAEPMGEKPV |
| Specificity | Human BANF1 has one isoform (89aa, 10kD). Mouse BANF1 has one isoform (89aa, 10kD) and Rat BANF1 also has one isoform (89aa, 10kD). NBP1-76751 can detect human, mouse and rat. |
| Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BANF1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Theoretical MW | 12 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
||
| Control Peptide |
|
||
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
| Publication using NBP1-76751 | Applications | Species |
|---|---|---|
| Tajik A, Zhang Y, Wei F et al. Transcription upregulation via force-induced direct stretching of chromatin. Nat Mater. 2016-08-22 [PMID: 27548707] (WB, Hamster) | WB | Hamster |
Secondary Antibodies |
Isotype Controls |
Research Areas for BANF1 Antibody (NBP1-76751)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: BANF1 Antibody [NBP1-76751] - Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/BANF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76751-img0002.jpg "Immunocytochemistry/Immunofluorescence: BANF1 Antibody [NBP1-76751] - Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 ug/ml.")
![Western Blot: BANF1 Antibody [NBP1-76751] - Analysis in Hela cell lysate with antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0003.jpg "Western Blot: BANF1 Antibody [NBP1-76751] - Analysis in Hela cell lysate with antibody at 1 ug/mL.")
![Western Blot: BANF1 Antibody [NBP1-76751] - Independent Antibody Validation via Protein Expression in Human Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1 [NBP1-76750] (2 ug/ml), BANF1 [NBP1-76751] (2 ug/ml) and beta-actin (1.5 ug/ml). 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0004.jpg "Western Blot: BANF1 Antibody [NBP1-76751] - Independent Antibody Validation via Protein Expression in Human Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1 [NBP1-76750] (2 ug/ml), BANF1 [NBP1-76751] (2 ug/ml) and beta-actin (1.5 ug/ml). 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76751-img0005.jpg "Western Blot: BANF1 Antibody [NBP1-76751] - Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1, NBP1-76751 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/ Immunofluorescence: BANF1 Antibody - BSA Free [NBP1-76751] - Figure 5 Immunofluorescence Validation of BANF1 in Human Kideny TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76751_rabbit-polyclonal-banf1-antibody-2711202420432380.jpg "Immunocytochemistry/ Immunofluorescence: BANF1 Antibody - BSA Free [NBP1-76751] - Figure 5 Immunofluorescence Validation of BANF1 in Human Kideny TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).")
![Immunocytochemistry/ Immunofluorescence: BANF1 Antibody - BSA Free [NBP1-76751] - Figure 5 Immunofluorescence Validation of BANF1 in Human Kideny TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76751_rabbit-polyclonal-banf1-antibody-2711202420324830.jpg "Immunocytochemistry/ Immunofluorescence: BANF1 Antibody - BSA Free [NBP1-76751] - Figure 5 Immunofluorescence Validation of BANF1 in Human Kideny TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).")
![Western Blot Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Western_Blot_5829.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11663.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11664.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot Erbin Antibody [Unconjugated]](https://images.novusbio.com/images2/Erbin_AF7866_Western_Blot_12827.jpg)
![Immunocytochemistry Erbin Antibody [Unconjugated]](https://images.novusbio.com/images2/Erbin_AF7866_Immunocytochemistry_12856.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")