![Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.](http://images.novusbio.com/fullsize/LEMD3-Antibody-OTI6C10-Western-Blot-NBP2-02211-img0007.jpg "Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | OTI6C10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.56 mg/ml |
| Immunogen | Full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LEMD3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 100 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.56 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for LEMD3 Antibody (NBP2-02211)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3.](http://images.novusbio.com/fullsize/LEMD3-Antibody-OTI6C10-Immunocytochemistry-Immunofluorescence-NBP2-02211-img0006.jpg "Immunocytochemistry/Immunofluorescence: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3.")
![Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of paraffin-embedded liver tissue using anti-LEMD3mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LEMD3-Antibody-OTI6C10-Immunohistochemistry-Paraffin-NBP2-02211-img0005.jpg "Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of paraffin-embedded liver tissue using anti-LEMD3mouse monoclonal antibody.")
![Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Analysis of Jurkat cells, using anti-LEMD3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/LEMD3-Antibody-OTI6C10-Flow-Cytometry-NBP2-02211-img0002.jpg "Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Analysis of Jurkat cells, using anti-LEMD3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).")
![Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.](http://images.novusbio.com/fullsize/LEMD3-Antibody-OTI6C10-Western-Blot-NBP2-02211-img0003.jpg "Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.")
![Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-LEMD3mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LEMD3-Antibody-OTI6C10-Immunohistochemistry-Paraffin-NBP2-02211-img0004.jpg "Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-LEMD3mouse monoclonal antibody.")
![Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Analysis of Hela cells, using anti-LEMD3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/LEMD3-Antibody-OTI6C10-Flow-Cytometry-NBP2-02211-img0001.jpg "Flow Cytometry: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Analysis of Hela cells, using anti-LEMD3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).")
![Immunoprecipitation: LEMD3 Antibody (OTI6C10) [NBP2-02211] - (Negative control: IP without adding anti-LEMD3 antibody). For each experiment, 500ul of DDK tagged LEMD3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LEMD3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/LEMD3-Antibody-OTI6C10-Immunoprecipitation-NBP2-02211-img0008.jpg "Immunoprecipitation: LEMD3 Antibody (OTI6C10) [NBP2-02211] - (Negative control: IP without adding anti-LEMD3 antibody). For each experiment, 500ul of DDK tagged LEMD3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LEMD3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")