![Western Blot: BANF1 Antibody [NBP1-76750] - Independent Antibody Validation via Protein Expression in Human Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1 [NBP1-76750] (2 ug/ml), BANF1 [NBP1-76751] (2 ug/ml) and beta-actin (1.5 ug/ml). 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76750-img0003.jpg "Western Blot: BANF1 Antibody [NBP1-76750] - Independent Antibody Validation via Protein Expression in Human Lines. Loading: 15 ug of lysates per lane. Antibodies: BANF1 [NBP1-76750] (2 ug/ml), BANF1 [NBP1-76751] (2 ug/ml) and beta-actin (1.5 ug/ml). 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human BANF1. The immunogen is located within the last 50 amino acids of BANF1. Amino Acid Sequence: LKKDEDLFREWLKDT |
| Specificity | Human BANF1 has one isoform (89aa, 10kD). Mouse BANF1 has one isoform (89aa, 10kD) and Rat BANF1 also has one isoform (89aa, 10kD). NBP1-76750 can detect human, mouse and rat. |
| Predicted Species | Mouse (100%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BANF1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 12 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for BANF1 Antibody (NBP1-76750)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: BANF1 Antibody [NBP1-76750] - Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/BANF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76750-img0002.jpg "Immunocytochemistry/Immunofluorescence: BANF1 Antibody [NBP1-76750] - Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 ug/ml.")
![Western Blot: BANF1 Antibody [NBP1-76750] - Human kidney tissue lysate with BANF1 antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/BANF1-Antibody-Western-Blot-NBP1-76750-img0001.jpg "Western Blot: BANF1 Antibody [NBP1-76750] - Human kidney tissue lysate with BANF1 antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: BANF1 Antibody - BSA Free [NBP1-76750] - Figure 2 Western Blot Validation in Human Kidney Tissue LysateLoading: 15 u of lysates per lane.Antibodies: BANF1 (A: 0.5 u/mL and B: 1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76750_rabbit-polyclonal-banf1-antibody-2711202419562191.jpg "Immunocytochemistry/ Immunofluorescence: BANF1 Antibody - BSA Free [NBP1-76750] - Figure 2 Western Blot Validation in Human Kidney Tissue LysateLoading: 15 u of lysates per lane.Antibodies: BANF1 (A: 0.5 u/mL and B: 1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/ Immunofluorescence: BANF1 Antibody - BSA Free [NBP1-76750] - Figure 2 Western Blot Validation in Human Kidney Tissue LysateLoading: 15 u of lysates per lane.Antibodies: BANF1 (A: 0.5 u/mL and B: 1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76750_rabbit-polyclonal-banf1-antibody-271120242013271.jpg "Immunocytochemistry/ Immunofluorescence: BANF1 Antibody - BSA Free [NBP1-76750] - Figure 2 Western Blot Validation in Human Kidney Tissue LysateLoading: 15 u of lysates per lane.Antibodies: BANF1 (A: 0.5 u/mL and B: 1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
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![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11664.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")