![Immunocytochemistry/Immunofluorescence: BANF1 Antibody (JG40-43) [NBP2-76978] - ICC staining BANF1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/BANF1-Antibody-JG40-43-Immunocytochemistry-Immunofluorescence-NBP2-76978-img0001.jpg "Immunocytochemistry/Immunofluorescence: BANF1 Antibody (JG40-43) [NBP2-76978] - ICC staining BANF1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | JG40-43 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human BANF1 aa 1-50 / 89. (SwissProt: O75531 Human; SwissProt: O54962 Mouse; SwissProt: Q9R1T1 Rat) |
| Localization | Nucleus. Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | BANF1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 10 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for BANF1 Antibody (NBP2-76978)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BANF1 |
![Immunohistochemistry-Paraffin: BANF1 Antibody (JG40-43) [NBP2-76978] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BANF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/BANF1-Antibody-JG40-43-Immunohistochemistry-Paraffin-NBP2-76978-img0009.jpg "Immunohistochemistry-Paraffin: BANF1 Antibody (JG40-43) [NBP2-76978] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BANF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: BANF1 Antibody (JG40-43) [NBP2-76978] - Flow cytometric analysis of MCF-7 cells with BANF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/BANF1-Antibody-JG40-43-Flow-Cytometry-NBP2-76978-img0007.jpg "Flow Cytometry: BANF1 Antibody (JG40-43) [NBP2-76978] - Flow cytometric analysis of MCF-7 cells with BANF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunohistochemistry: BANF1 Antibody (JG40-43) [NBP2-76978] - Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-BANF1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BANF1-Antibody-JG40-43-Immunohistochemistry-NBP2-76978-img0002.jpg "Immunohistochemistry: BANF1 Antibody (JG40-43) [NBP2-76978] - Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-BANF1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry: BANF1 Antibody (JG40-43) [NBP2-76978] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-BANF1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BANF1-Antibody-JG40-43-Immunohistochemistry-NBP2-76978-img0003.jpg "Immunohistochemistry: BANF1 Antibody (JG40-43) [NBP2-76978] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-BANF1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry: BANF1 Antibody (JG40-43) [NBP2-76978] - Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-BANF1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BANF1-Antibody-JG40-43-Immunohistochemistry-NBP2-76978-img0004.jpg "Immunohistochemistry: BANF1 Antibody (JG40-43) [NBP2-76978] - Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-BANF1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry: BANF1 Antibody (JG40-43) [NBP2-76978] - Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-BANF1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BANF1-Antibody-JG40-43-Immunohistochemistry-NBP2-76978-img0005.jpg "Immunohistochemistry: BANF1 Antibody (JG40-43) [NBP2-76978] - Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-BANF1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry: BANF1 Antibody (JG40-43) [NBP2-76978] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BANF1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/BANF1-Antibody-JG40-43-Immunohistochemistry-NBP2-76978-img0006.jpg "Immunohistochemistry: BANF1 Antibody (JG40-43) [NBP2-76978] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BANF1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: BANF1 Antibody (JG40-43) [NBP2-76978] - Analysis of paraffin-embedded human thyroid tissue using anti-BANF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/BANF1-Antibody-JG40-43-Immunohistochemistry-Paraffin-NBP2-76978-img0008.jpg "Immunohistochemistry-Paraffin: BANF1 Antibody (JG40-43) [NBP2-76978] - Analysis of paraffin-embedded human thyroid tissue using anti-BANF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: BANF1 Antibody (JG40-43) [NBP2-76978] - Analysis of paraffin-embedded human thyroid carcinoma tissue using anti-BANF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/BANF1-Antibody-JG40-43-Immunohistochemistry-Paraffin-NBP2-76978-img0010.jpg "Immunohistochemistry-Paraffin: BANF1 Antibody (JG40-43) [NBP2-76978] - Analysis of paraffin-embedded human thyroid carcinoma tissue using anti-BANF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Western_Blot_5829.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11663.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11664.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot Erbin Antibody [Unconjugated]](https://images.novusbio.com/images2/Erbin_AF7866_Western_Blot_12827.jpg)
![Immunocytochemistry Erbin Antibody [Unconjugated]](https://images.novusbio.com/images2/Erbin_AF7866_Immunocytochemistry_12856.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")