![Simple Western: BAF53A Antibody [NB100-61628] - Simple Western lane view shows a specific band for BAF53A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Simple-Western-NB100-61628-img0007.jpg "Simple Western: BAF53A Antibody [NB100-61628] - Simple Western lane view shows a specific band for BAF53A in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC, IP (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human BRG1-associated factor, 53 kDa subunit A using the numbering given in entry NP_004292.1 (GeneID 86). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ACTL6A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF reactivity reported in (PMID: 23395444). Use in IHC reported in scientific literature (PMID:33469301) |
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| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BAF53A Antibody (NB100-61628)Find related products by research area.
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![Western Blot: BAF53A Antibody [NB100-61628] - Western blot of WT and ARID1A-/- HCT116 nuclear extracts separated by glycerol gradient centrifugation. BAF53A antibody (NB100-61628) used for blotting. Representative of three biological replicates. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/30506#content) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-61628-img0038.jpg "Western Blot: BAF53A Antibody [NB100-61628] - Western blot of WT and ARID1A-/- HCT116 nuclear extracts separated by glycerol gradient centrifugation. BAF53A antibody (NB100-61628) used for blotting. Representative of three biological replicates. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/30506#content) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunohistochemistry-Paraffin-NB100-61628-img0035.jpg "Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB")
![Western Blot: BAF53A Antibody [NB100-61628] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-BAF53A antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/BAF53A-Antibody-Western-Blot-NB100-61628-img0036.jpg "Western Blot: BAF53A Antibody [NB100-61628] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-BAF53A antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BAF53A-Antibody-Immunohistochemistry-Paraffin-NB100-61628-img0034.jpg "Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:200 (1ug/ml). Detection: DAB")
![Western Blot: BAF53A Antibody [NB100-61628] - BRD9 defines a distinct, non-canonical BAF complex. a Immunoprecipitation (IP)-mass spectrometry using BRD9 or IgG antibody from mouse ESCs. The plot shows the spectral count fold change in BRD9 IP relative to IgG & the corresponding AC test p values, calculated with two technical replicates using PatternLab. In orange are proteins that satisfy FC 2, AC test p < 0.05. b Immunoblotting analysis of fractions after mouse ESC nuclear lysates were subjected to a density sedimentation assay in 10–30% glycerol gradient. LMW & HMW indicate lower & higher molecular weights, respectively. Nonspecific bands are marked with an asterisk. Molecular weights from ladder are indicated. c Immunoprecipitation (IP) experiments from mouse ESC nuclear lysates using antibodies against BRG1, BRD9, & BAF47. Blots developed using chemiluminescence are marked with double asterisks; each IP was taken from a different exposure. d Depletion IP experiment from ESCs using antibodies against BRG1 or BAF155. Each lane shows the remaining proteins after each successive IP, labeled 1 through 4. e IP experiment from ESCs using antibodies against IgG or BRD9, which was incubated in increasing concentration of urea. f Schematic of the esBAF, GBAF, & PBAF complexes in mouse ESCs. Colored in orange, pink, & mustard yellow are subunits that define each individual complex. In green is the enzymatic component, BRG1. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30510198), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-61628_rabbit-polyclonal-baf53a-antibody-310202415532068.jpg "Western Blot: BAF53A Antibody [NB100-61628] - BRD9 defines a distinct, non-canonical BAF complex. a Immunoprecipitation (IP)-mass spectrometry using BRD9 or IgG antibody from mouse ESCs. The plot shows the spectral count fold change in BRD9 IP relative to IgG & the corresponding AC test p values, calculated with two technical replicates using PatternLab. In orange are proteins that satisfy FC 2, AC test p < 0.05. b Immunoblotting analysis of fractions after mouse ESC nuclear lysates were subjected to a density sedimentation assay in 10–30% glycerol gradient. LMW & HMW indicate lower & higher molecular weights, respectively. Nonspecific bands are marked with an asterisk. Molecular weights from ladder are indicated. c Immunoprecipitation (IP) experiments from mouse ESC nuclear lysates using antibodies against BRG1, BRD9, & BAF47. Blots developed using chemiluminescence are marked with double asterisks; each IP was taken from a different exposure. d Depletion IP experiment from ESCs using antibodies against BRG1 or BAF155. Each lane shows the remaining proteins after each successive IP, labeled 1 through 4. e IP experiment from ESCs using antibodies against IgG or BRD9, which was incubated in increasing concentration of urea. f Schematic of the esBAF, GBAF, & PBAF complexes in mouse ESCs. Colored in orange, pink, & mustard yellow are subunits that define each individual complex. In green is the enzymatic component, BRG1. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30510198), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")