![Western Blot: BACE-1 Antibody [NBP1-77307] - IAV via Protein Expression Profile in Cell Lines. Antibodies: BACE, beta-actin, and GAPDH. Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/BACE-1-Antibody-Western-Blot-NBP1-77307-img0007.jpg "Western Blot: BACE-1 Antibody [NBP1-77307] - IAV via Protein Expression Profile in Cell Lines. Antibodies: BACE, beta-actin, and GAPDH. Secondary: Goat anti-rabbit IgG HRP conjugate.")
| Reactivity | Hu, Mu, Rt, Bv, GpSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BACE. The immunogen is located within the last 50 amino acids of BACE. Amino Acid Squence: CLRQQHDDFADDISLL |
| Specificity | Human BACE-1 has 6 isoforms, including isoform A (501aa, 55.8kD), isoform B (476aa, 52.9kD), isoform C (457aa, 51.1kD), isoform D (432aa, 48.2kD), isoform 5 (401aa, 45kD) and isoform 6 (376aa, 42.2kD). This antibody detects all human isoforms. Mouse BACE-1 has only 1 isoform (501aa, 55.7kD). Rat BACE-1 has only one isoform identified so far (501aa, 55.8kD). |
| Predicted Species | Rat (94%), Bovine (94%), Guinea Pig (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BACE1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
||
| Control Peptide |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BACE-1 Antibody (NBP1-77307)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry: BACE-1 Antibody [NBP1-77307] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/BACE-1-Antibody-Immunohistochemistry-NBP1-77307-img0004.jpg "Immunohistochemistry: BACE-1 Antibody [NBP1-77307] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.")
![Western Blot: BACE-1 Antibody [NBP1-77307] - Analysis of BACE in human brain tissue lysate in the absence (A) or presence (B) of blocking peptide (2253P) and in mouse 3T3 cell lysate (C) with anti-BACE (CT) using NBP1-77307 at 1 ug/ml.](http://images.novusbio.com/fullsize/BACE-1-Antibody-Western-Blot-NBP1-77307-img0003.jpg "Western Blot: BACE-1 Antibody [NBP1-77307] - Analysis of BACE in human brain tissue lysate in the absence (A) or presence (B) of blocking peptide (2253P) and in mouse 3T3 cell lysate (C) with anti-BACE (CT) using NBP1-77307 at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody - BSA Free [NBP1-77307] - Figure 4 Immunofluorescence Validation of BACE-1 in 3T3/NIH CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse 3T3/NIH cells labeling BACE-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing both membrane and cytosol staining on 3T3/NIH cells.](http://images.novusbio.com/fullsize/nbp1-77307_rabbit-polyclonal-bace-1-antibody-2711202419592054.gif "Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody - BSA Free [NBP1-77307] - Figure 4 Immunofluorescence Validation of BACE-1 in 3T3/NIH CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse 3T3/NIH cells labeling BACE-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing both membrane and cytosol staining on 3T3/NIH cells.")
![Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody - BSA Free [NBP1-77307] - Figure 5 Immunocytochemistry Validation of BACE-1 in 3T3/NIH CellsImmunocytochemical analysis of 3T3/NIH cells using anti-BACE-1 antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77307_rabbit-polyclonal-bace-1-antibody-2711202420392792.jpg "Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody - BSA Free [NBP1-77307] - Figure 5 Immunocytochemistry Validation of BACE-1 in 3T3/NIH CellsImmunocytochemical analysis of 3T3/NIH cells using anti-BACE-1 antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![ADAM10 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "ADAM10 [Unconjugated]")
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neprilysin_AF1126_Immunohistochemistry_6679.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-8220249505976.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-44202415433122.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")