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Images
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # ...read more
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # ...read more
B220/CD45R was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B220/CD45R Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1217G; green) at 5 µg/mL for 3 hours at room ...read more
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for B220/CD45R Antibody (RA3-6B2) [Alexa Fluor® 488]
B220
CD45 antigen
CD45R
EC 3.1.3.48
L-CA
LY5
protein tyrosine phosphatase, receptor type, C
receptor-type tyrosine-protein phosphatase C
T200 glycoprotein
T200 leukocyte common antigen
T200receptor type, c polypeptide
Background
B220 (accession # P06800) is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
Coffman, R.L. (1982) Immuno. Rev. 69:5.
Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
Allman, D.M. et al. (1992) J. Immunol. 149:2533.
Asensi, V.K. et al. (1989) Immunology 68:204.
Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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