Home » B220/CD45R » B220/CD45R Antibodies » B220/CD45R Antibody (RA3-6B2) [Fluorescein]
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B220/CD45R Antibody (RA3-6B2) [Fluorescein]

 (3 Publications)
 (1 Review)
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Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F) or ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
RA3-6B2
Clonality
Monoclonal
Host
Rat
Conjugate
Fluorescein

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1217U-100UG
Alexa Fluor 405
FAB1217V-100
Alexa Fluor 405
FAB1217V-025
Alexa Fluor 488
FAB1217G
Alexa Fluor 532
FAB1217X
Alexa Fluor 594
FAB1217T-100UG
Alexa Fluor 647
FAB1217R-100UG
Alexa Fluor 700
FAB1217N-025
Alexa Fluor 750
FAB1217S-100UG
Allophycocyanin
FAB1217A
Allophycocyanin/Cy7
FAB1217APCCY7
Biotin
BAM1217
DyLight 350
FAB1217D
DyLight 405
FAB1217E
DyLight 488
FAB1217K
DyLight 550
FAB1217L
DyLight 594
FAB1217M
DyLight 650
FAB1217W
DyLight 680
FAB1217Y
DyLight 755
FAB1217Z
HRP
FAB1217H
Janelia Fluor 549
FAB1217I
Janelia Fluor 646
FAB1217J
PE/Atto594
FAB1217PEATT594
PE/Cy5.5
FAB1217PECY55
PE/Cy7
FAB1217PECY7
Phycoerythrin
FAB1217P
Unconjugated
BAM1217
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
BAM1217
Carrier Free
FAB1217P
Carrier Free
FAB1217A
Carrier Free
FAB1217G
Carrier Free
FAB1217N-025
Carrier Free
FAB1217V-100
Carrier Free
FAB1217V-025
Carrier Free
FAB1217U-100UG
Carrier Free
FAB1217S-100UG
Carrier Free
FAB1217T-100UG
Carrier Free
FAB1217R-100UG
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B220/CD45R Antibody (RA3-6B2) [Fluorescein] Summary

Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Specificity
Detects mouse B220/CD45R.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
PTPRC
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 1 Review rated 5
using
FAB1217F in the following application:
Publications
Read Publications using
FAB1217F in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B220/CD45R Antibody (RA3-6B2) [Fluorescein]

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

  1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
  2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
  3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
  4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
  5. Asensi, V.K. et al. (1989) Immunology 68:204.
  6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B220/CD45R Antibody (FAB1217F)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(2)
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Mouse
(2)
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Review for B220/CD45R Antibody (FAB1217F) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: IL1-R KO Mouse.

Reviews using FAB1217F:
Filter by Applications
Flow
(1)
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Filter by Species
IL1-R KO Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry B220/CD45R FAB1217F
Enlarge 		5
reviewed by:
James Xiao
 Flow IL1-R KO Mouse 01/03/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedMouse spleen cells
SpeciesIL1-R KO Mouse
LotLCB0213081

Comments

CommentsThe single cell suspension was isolated from a spleen of an IL1-R KO (knockout) mouse and stained with the FITC-Conjugated CD45R antibody (1:100) for 30 minutes. Cells were analyzed by flow cytometer.

Product General Protocols

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FAQs for B220/CD45R Antibody (FAB1217F) (0)

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Secondary Antibodies

 

Isotype Controls

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Array FAB1217F

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Recent Reviews

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5
1
4
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1
0
Flow Cytometry B220/CD45R FAB1217F
Enlarge
 James Xiao
01/03/2018
Application: Flow
Species: IL1-R KO Mouse
read this review

Bioinformatics

Gene Symbol PTPRC