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B220/CD45R Antibody (RA3-6B2) [Biotin]

 (3 Publications)
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Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated B220/CD45R Monoclonal Antibody (Catalog # BAM1217) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.
Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated B220/CD45R Monoclonal Antibody (Catalog # BAM1217) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by ...read more
B220/CD45R was detected in immersion fixed mouse splenocytes using Mouse B220/CD45R Biotinylated Monoclonal Antibody (Catalog # BAM1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IP, ICC/IF
Clone
RA3-6B2
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1217U-100UG
Alexa Fluor 405
FAB1217V-100
Alexa Fluor 405
FAB1217V-025
Alexa Fluor 488
FAB1217G
Alexa Fluor 532
FAB1217X
Alexa Fluor 594
FAB1217T-100UG
Alexa Fluor 647
FAB1217R-100UG
Alexa Fluor 700
FAB1217N-025
Alexa Fluor 750
FAB1217S-100UG
Allophycocyanin
FAB1217A
Allophycocyanin/Cy7
FAB1217APCCY7
DyLight 350
FAB1217D
DyLight 405
FAB1217E
DyLight 488
FAB1217K
DyLight 550
FAB1217L
DyLight 594
FAB1217M
DyLight 650
FAB1217W
DyLight 680
FAB1217Y
DyLight 755
FAB1217Z
Fluorescein
FAB1217F
HRP
FAB1217H
Janelia Fluor 549
FAB1217I
Janelia Fluor 646
FAB1217J
PE/Atto594
FAB1217PEATT594
PE/Cy5.5
FAB1217PECY55
PE/Cy7
FAB1217PECY7
Phycoerythrin
FAB1217P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB1217F
Carrier Free
FAB1217P
Carrier Free
FAB1217A
Carrier Free
FAB1217G
Carrier Free
FAB1217N-025
Carrier Free
FAB1217V-100
Carrier Free
FAB1217V-025
Carrier Free
FAB1217U-100UG
Carrier Free
FAB1217S-100UG
Carrier Free
FAB1217T-100UG
Carrier Free
FAB1217R-100UG
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B220/CD45R Antibody (RA3-6B2) [Biotin] Summary

Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Specificity
Detects mouse B220/CD45R. Reacts with the extracellular domain of mouse B220/CD45R antigen.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
PTPRC
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation
Readout System
Publications
Read Publications using
BAM1217 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B220/CD45R Antibody (RA3-6B2) [Biotin]

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

B220 (accession # P06800) is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

  1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
  2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
  3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
  4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
  5. Asensi, V.K. et al. (1989) Immunology 68:204.
  6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B220/CD45R Antibody (BAM1217)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Flow Cytometry, ICC, IHC-Fr.


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Flow Cytometry
(1)
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(1)
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(1)
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(3)
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Bioinformatics

Gene Symbol PTPRC