Reactivity | HuSpecies Glossary |
Applications | WB, Flow, ICC/IF, IHC |
Clone | PTPRC/1783R |
Clonality | Monoclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Description | 200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54578) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
Additional Information | Recombinant Monoclonal Antibody. |
Immunogen | Recombinant human full-length B220/CD45R protein (Uniprot: P08575 ) |
Localization | Cell Surface and Cytoplasmic |
Marker | B-Cell Marker |
Isotype | IgG |
Clonality | Monoclonal |
Host | Rabbit |
Gene | PTPRC |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein A purified |
Publication using NBP2-53303 | Applications | Species |
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Atif SM, Mack DG, McKee AS, Rangel-Moreno J et al. Protective role of B cells in sterile particulate-induced lung injury JCI Insight 2019-05-16 [PMID: 31094704] (IHC-P, Mouse) | IHC-P | Mouse |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-P | Human | 10/13/2020 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for B220/CD45R Antibody (NBP2-53303)Find related products by research area.
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