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B220/CD45R Antibody (PTPRC/1783R)

Images

 
Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).
Immunocytochemistry/ Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Paraformaldehyde-fixed Raji cells stained with B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Green). ...read more
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - FFPE section of human lung cancer. 10X magnification. IHC-P image submitted by a verified customer review.
Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Flow Cytometric Analysis of PFA-fixed Raji cells. B220/CD45R antibody (PTPRC/1783R) followed by goat anti-rabbit IgG-CF488 (blue); Isotype Control (red).
Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Formalin-fixed paraffin-embedded human Tonsil stained with CD45RB Recombinant Rabbit Monoclonal Antibody (PTPRC/1783R).
Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Blue); ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
PTPRC/1783R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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B220/CD45R Antibody (PTPRC/1783R) Summary

Description
200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54578)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant human full-length B220/CD45R protein (Uniprot: P08575 )
Localization
Cell Surface and Cytoplasmic
Marker
B-Cell Marker
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PTPRC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-53303 in the following applications:

Publications
Read Publication using
NBP2-53303 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for B220/CD45R Antibody (PTPRC/1783R)

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B220/CD45R Antibody (NBP2-53303)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Review for B220/CD45R Antibody (NBP2-53303) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-53303:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin B220/CD45R NBP2-53303
Enlarge
4
reviewed by:
Verified Customer
IHC-P Human 10/13/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFFPE,human lung cancer
SpeciesHuman
Lot5588-23P190209

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for B220/CD45R Antibody (NBP2-53303) (0)

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Recent Reviews

4
5
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4
1
3
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2
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1
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Verified Customer
10/13/2020
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol PTPRC
Uniprot