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ATP6V1H Antibody

Images

 
Western Blot: ATP6V1H Antibody [NBP1-85668] - Analysis in control (vector only transfected HEK293T lysate) and ATP6V1H over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: ATP6V1H Antibody [NBP1-85668] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ATP6V1H Antibody [NBP1-85668] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ATP6V1H Antibody [NBP1-85668] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ATP6V1H Antibody [NBP1-85668] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: ATP6V1H Antibody [NBP1-85668] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ATP6V1H Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PQVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSE
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V1H
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 26168401). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ATP6V1H Protein (NBP1-85668PEP)
Publications
Read Publication using
NBP1-85668 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26168401).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATP6V1H Antibody

  • ATPase, H+ transporting, lysosomal 50/57kD, V1 subunit H
  • ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
  • CGI-11
  • MSTP042
  • NBP1
  • Nef-binding protein 1
  • Protein VMA13 homolog
  • SFD
  • SFDalpha
  • SFDbeta
  • vacuolar ATP synthase subunit H
  • vacuolar ATPase subunit H
  • vacuolar proton pump H subunit
  • Vacuolar proton pump subunit H
  • Vacuolar proton pump subunit SFD
  • V-ATPase 50/57 kDa subunits
  • V-ATPase H subunit
  • V-ATPase subunit H
  • VMA13
  • V-type proton ATPase subunit H

Background

ATP6V1H, also known as V-type proton ATPase subunit H, is a 483 amino acid protein that is a subunit of the V1 complex, which is responsible for acidifying eukaryotic cells in intracellular organelles. Studies on this protein have shown a relationship with the following disorders and diseases, osteopetrosis, carcinoma, cholera, colon carcinoma, arthritis, rheumatoid arthritis, neuronitis, tuberculosis, and immunodeficiency. The ATP6V1H protein has also shown an interaction with ATP6V1D, ATP6V1F, ATP6V1B2, ATP6V1E1, and ETHE1 in the oxidative phosphorylation, metabolic, phagosome, lysosome, insulin receptor recycline, and Iron uptake and transport pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATP6V1H Antibody (NBP1-85668)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ATP6V1H