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AP2M1 Antibody (OTI1E9)

Images

 
Western Blot: AP2M1 Antibody (1E9) [NBP2-00834] Analysis of extracts (35ug) from 9 different cell lines by using anti-AP2M1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: AP2M1 Antibody (1E9) [NBP2-00834] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AP2M1.
Immunohistochemistry-Paraffin: AP2M1 Antibody (1E9) [NBP2-00834] - Staining of paraffin-embedded Human colon tissue using anti-AP2M1 mouse monoclonal antibody.
Western Blot: AP2M1 Antibody (1E9) [NBP2-00834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.65 mg/ml

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AP2M1 Antibody (OTI1E9) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 97-383 of human AP2M1(NP_001020376) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
AP2M1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200-500
Theoretical MW
49.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-00834 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.65 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AP2M1 Antibody (OTI1E9)

  • Adaptin-mu2
  • adaptor-related protein complex 2, mu 1 subunit
  • AP-2 mu chain
  • Clathrin assembly protein complex 2 medium chain
  • clathrin coat adaptor protein AP50
  • Clathrin coat assembly protein AP50
  • Clathrin coat-associated protein AP50
  • clathrin-associated/assembly/adaptor protein, medium 1
  • HA2 50 kDa subunit
  • KIAA0109
  • mu subunit
  • Plasma membrane adaptor AP-2 50 kDa protein
  • plasma membrane adaptor AP-2 50kDA protein

Background

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AP2M1 Antibody (NBP2-00834)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AP2M1 Antibody (NBP2-00834). (Showing 1 - 1 of 1 FAQ).

  1. I have a question about your product ,named AP2M1 antibody (1E9) NBP2-00834. I didn't find the concentration of this anbody on the product page, could you please tell me the concentrarion infomation of this antibody?
    • Current lot of AP2M1 antibody NBP2-00834 is supplied at 0.65 mg/ml. The vial is 100ul.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AP2M1
Entrez