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ATP5A Antibody (1082626) [Unconjugated]

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ATP5A1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human ATP5A1 Monoclonal Antibody (Catalog # MAB11558) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of mitochondria. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ATP5A1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human ATP5A1 Monoclonal Antibody (Catalog # MAB11558) at 5 µg/ml for 1 hour at room temperature followed by incubation ...read more
ATP5A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human ATP5A1 Monoclonal Antibody (Catalog # MAB11558) at 8 µg/ml for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, ICC/IF
Clone
1082626
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB11558-SP
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ATP5A Antibody (1082626) [Unconjugated] Summary

Immunogen
ATP5A1 contatining synthetic peptide
Accession # P25705
Specificity
Detects a synthetic peptide around aa 235 in Direct ELISA
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Instructions
Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ATP5A Antibody (1082626) [Unconjugated]

  • ATP synthase alpha chain, mitochondrial
  • ATP synthase subunit alpha, mitochondrial
  • ATP synthase, H+ transporting, mitochondrial F1 co
  • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
  • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle
  • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2
  • ATP sythase (F1-ATPase) alpha subunit
  • ATP5A
  • ATP5A1
  • ATP5AL2
  • ATP5AMOM2
  • ATPM
  • ATPMATP synthase subunit alpha, mitochondrial
  • cardiac muscle
  • COXPD22
  • EC 3.6.3
  • EC 3.6.3.14
  • epididymis secretory sperm binding protein Li 123m
  • hATP1
  • HEL-S-123m
  • MC5DN4
  • MC5DN4A
  • MC5DN4B
  • mitochondrial ATP synthetase, oligomycin-resistant
  • MOM2
  • OMR
  • ORM
  • ORMATP synthase alpha chain, mitochondrial

Background

ATP5F1A is a 553aa, 60kDa protein which encodes subunit alpha of mitochondrial ATP synthase (complex V), the rotary ATPase responsible for more than 90% of cellular ATP synthesis.  F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c. ATP5A1 is generally regarded to mediate tumor progression through mitochondrial signaling.  It is overrepresented in an oxidative phosphorylation pathway associated with tumorigenesis and tumor progression. Dysregulation of this ATP synthase subunit is observed in human carcinomas.
  1. Lines MA, Cuillerier A, Chakraborty P, Naas T, Duque Lasio ML, Michaud J, Pileggi C, Harper ME, Burelle Y, Toler TL, Sondheimer N, Crawford HP, Millan F, Geraghty MT. A recurrent de novo ATP5F1A substitution associated with neonatal complex V deficiency. Eur J Hum Genet. 2021 Nov;29(11):1719-1724. doi: 10.1038/s41431-021-00956-0. Epub 2021 Sep 6. PMID: 34483339; PMCID: PMC8560863.
  2. Song Ba Y, Wang Ma F, Wei Ma Y, Chen Ba D, Deng Ba G. ATP5A1 Participates in Transcriptional and Posttranscriptional Regulation of Cancer-Associated Genes by Modulating Their Expression and Alternative Splicing Profiles in HeLa Cells. Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211039126. doi: 10.1177/15330338211039126. PMID: 34520292; PMCID: PMC8445539.
  3. Yuan L, Chen L, Qian K, Wang G, Lu M, Qian G, Cao X, Jiang W, Xiao Y, Wang X. A novel correlation between ATP5A1 gene expression and progression of human clear cell renal cell carcinoma identified by co‑expression analysis. Oncol Rep. 2018 Feb;39(2):525-536. doi: 10.3892/or.2017.6132. Epub 2017 Dec 4. PMID: 29207195; PMCID: PMC5783621.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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