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ATP2C1 Antibody (2G1)

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Western Blot: ATP2C1 Antibody (2G1) [H00027032-M01] - ATP2C1 monoclonal antibody (M01), clone 2G1 Analysis of ATP2C1 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of monoclonal antibody to ATP2C1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of ATP2C1 over-expressed 293 cell line, cotransfected with ATP2C1 Validated Chimera RNAi ( Cat # H00027032-R01V ) (Lane 2) or non-transfected control (Lane ...read more
Western Blot: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of ATP2C1 expression in transfected 293T cell line by ATP2C1 monoclonal antibody (M01), clone 2G1.Lane 1: ATP2C1 transfected lysate(100.6 KDa).Lane 2: ...read more
Immunoprecipitation: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of ATP2C1 transfected lysate using anti-ATP2C1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ATP2C1 MaxPab rabbit ...read more
ELISA: ATP2C1 Antibody (2G1) [H00027032-M01] - Detection limit for recombinant GST tagged ATP2C1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP, KD
Clone
2G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ATP2C1 Antibody (2G1) Summary

Immunogen
ATP2C1 (AAH28139, 119 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VQEYRSEKSLEELSKLVPPECHCVREGKLEHTLARDLVPGDTVCLSVGDRVPADLRLFEAVDLSIDESSLTGETTPCSKVTAPQPAATNGDLASRSNIAFMGTLVRCGKAKGVVIGTGENSEFGEVFKMMQAEEAPKTPLQKSMDLLGKQL
Specificity
ATP2C1 - ATPase, Ca++ transporting, type 2C, member 1
Isotype
IgG1 Lambda
Clonality
Monoclonal
Host
Mouse
Gene
ATP2C1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA.
Publications
Read Publications using
H00027032-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ATP2C1 Antibody (2G1)

  • ATPase, Ca++ transporting, type 2C, member 1
  • BCPM
  • benign chronic pemphigus (Hailey-Hailey disease)
  • Ca(2+)-sequestering
  • calcium-transporting ATPase type 2C member 1
  • EC 3.6.3
  • HHD
  • hSPCA1
  • HUSSY-28
  • PMR1L
  • secretory pathway Ca2+/Mn2+ ATPase 1

Background

The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATP2C1 Antibody (H00027032-M01)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATP2C1 Antibody (H00027032-M01) (0)

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Secondary Antibodies

 

Isotype Controls

Additional ATP2C1 Products

Array H00027032-M01

Research Areas for ATP2C1 Antibody (H00027032-M01)

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Blogs on ATP2C1

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Bioinformatics

Gene Symbol ATP2C1
Entrez
OMIM
Uniprot