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ATF2 Antibody

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse ATF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ATF2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF4455-SP
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ATF2 Antibody Summary

Immunogen
E. coli-derived recombinant human ATF2
Val270-Ser505
Accession # P15336
Specificity
Detects human and mouse ATF2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ATF2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ATF2 Antibody

  • activating transcription factor 2formerly
  • ATF2
  • cAMP responsive element binding protein 2
  • cAMP-responsive element-binding protein 2
  • CREB2
  • CREBP1
  • CRE-BP1
  • cyclic AMP-dependent transcription factor ATF-2
  • HB16
  • MGC111558
  • mXBP
  • TREB7

Background

ATF2 is a member of the bzip family of transcription factors. ATF2 responds to cAMP activation in cells by either homo or hetero dimerizing with c-Jun and binding to cAMP response elements (TGACGTCA). ATF2 also contains histone acetylase activity that can be induced by ionizing radiation. ATF2 can be phosphorylated by a variety of kinases (JNK, p38, ATM), and this phosphorylation correlates with increased activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ATF2
Uniprot