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ASGPR1 Antibody - BSA Free

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Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85580] - Analysis in human liver and endometrium tissues using NBP1-85580 antibody. Corresponding ASGPR1 RNA-seq data are presented for the same tissues.
Western Blot: ASGPR1 Antibody [NBP1-85580] - Analysis in human cell line U-2197.
Immunocytochemistry/ Immunofluorescence: ASGPR1 Antibody [NBP1-85580] - Staining of human cell line Hep G2 shows localization to cell junctions & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85580] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85580] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85580] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85580] - Staining of human tonsil shows no positivity in germinal center cells as expected.
Independent Antibodies: Staining of human endometrium, kidney, liver and tonsil using NBP1-85580 (A) shows similar protein distribution across tissues to independent antibody NBP1-85581 (B).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
 

Independent Antibodies

       

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Catalog# & Formulation Size Price

ASGPR1 Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASGR1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ASGPR1 Protein (NBP1-85580PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ASGPR1 Antibody - BSA Free

  • ASGPR 1
  • ASGP-R 1
  • ASGPR
  • ASGPR1
  • ASGR1
  • asialoglycoprotein receptor 1
  • CLEC4H1
  • CLEC4H1ASGPR1
  • C-type lectin domain family 4 member H1
  • Hepatic lectin H1
  • HL-1
  • MHL1
  • RHL1

Background

Partially deglycosylated plasma glycoproteins and immunoglobulin IgA2 allotypes are efficiently and specificallyremoved from circulation by a receptor-mediated process. The asialoglycoprotein receptor binds to desialylated(galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubulesto an acidic-sorting organelle where the receptor and ligand dissociate. Then the receptor is recycled back to thecell surface and the ligand is transported to the lysosomes for degradation. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ASGR1
Entrez
Uniprot