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APEH Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: APEH Antibody [NBP1-85333] - Staining in human kidney and pancreas tissues using anti-APEH antibody. Corresponding APEH RNA-seq data are presented for the ...read more
Independent Antibodies: Western Blot: APEH Antibody [NBP1-85333] - Analysis using Anti-APEH antibody NBP1-85333 (A) shows similar pattern to independent antibody NBP1-85332 (B).
Immunocytochemistry/ Immunofluorescence: APEH Antibody [NBP1-85333] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: APEH Antibody [NBP1-85333] - Staining of human kidney.
Immunohistochemistry-Paraffin: APEH Antibody [NBP1-85333] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: APEH Antibody [NBP1-85333] - Staining of human colon.
Immunohistochemistry-Paraffin: APEH Antibody [NBP1-85333] - Staining of human pancreas.
Immunohistochemistry-Paraffin: APEH Antibody [NBP1-85333] - Staining of human cerebral cortex.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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APEH Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IDQDLMVAQFSTPSLPPTLKVGFLPSAGKEQSVLWVSLEEAEPIPDIHWGIRVLQPPPEQENVQYAGLDFEAILLQPGSPPDKTQVP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APEH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 24554718). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
APEH Protein (NBP1-85333PEP)
Publications
Read Publication using
NBP1-85333 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%). Mouse reactivity reported in scientific literature (PMID: 24554718).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for APEH Antibody

  • AARE
  • ACPH
  • acylamino-acid-releasing enzyme
  • acylaminoacyl-peptidase
  • Acyl-peptide hydrolase
  • APH
  • D3F15S2
  • D3S48EEC 3.4.19.1
  • DNF15S2
  • MGC2178
  • N-acylaminoacyl-peptide hydrolase
  • OPH
  • Oxidized protein hydrolase

Background

The APEH gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated aminoacid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is furtherprocessed to acetate and a free

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for APEH Antibody (NBP1-85333)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Mouse
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for APEH Antibody (NBP1-85333) (0)

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Secondary Antibodies

 

Isotype Controls

Additional APEH Products

Array NBP1-85333

Research Areas for APEH Antibody (NBP1-85333)

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Blogs on APEH

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Bioinformatics

Gene Symbol APEH