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AP2 alpha Antibody (AP2a 8G8/5) - BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP
Clone
AP2a 8G8/5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.82 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2639AF350
Alexa Fluor 405
NB100-2639AF405
Alexa Fluor 488
NB100-2639AF488
Alexa Fluor 532
NB100-2639AF532
Alexa Fluor 594
NB100-2639AF594
Alexa Fluor 647
NB100-2639AF647
Alexa Fluor 700
NB100-2639AF700
Alexa Fluor 750
NB100-2639AF750
Biotin
NB100-2639B
CoraFluor 1
NB100-2639CL1
DyLight 350
NB100-2639UV
DyLight 405
NB100-2639V
DyLight 488
NB100-2639G
DyLight 550
NB100-2639R
DyLight 594
NB100-2639DL594
DyLight 650
NB100-2639C
DyLight 680
NB100-2639FR
DyLight 755
NB100-2639IR
FITC
NB100-2639F
HRP
NB100-2639H
Janelia Fluor 525
NB100-2639JF525
Janelia Fluor 549
NB100-2639JF549
Janelia Fluor 585
NB100-2639JF585
Janelia Fluor 635
NB100-2639JF635
Janelia Fluor 646
NB100-2639JF646
Janelia Fluor 669
NB100-2639JF669
mFluor Violet 450 SE
NB100-2639MFV450
mFluor Violet 500 SE
NB100-2639MFV500
mFluor Violet 610 SE
NB100-2639MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NB100-2639-0.025mg
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AP2 alpha Antibody (AP2a 8G8/5) - BSA Free Summary

Immunogen
Bacterialy expressed AP-2a/AP-2g fusion protein.
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TFAP2A
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Application Notes
Antigen recovery using microwave or pressure cooking required for paraffin embedded tissue. Positive control(s): Extract from breast cancer cell line ZR75-1.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Purity
Protein A purified

Alternate Names for AP2 alpha Antibody (AP2a 8G8/5) - BSA Free

  • Activating enhancer-binding protein 2-alpha
  • Activator protein 2
  • AP-2 transcription factor
  • AP2-alpha
  • AP2TFAP-2alpha
  • AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha)
  • BOFS
  • FLJ51761
  • TFAP2
  • transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
  • transcription factor AP-2-alpha

Background

Activation Protein transcription factor 2 (AP2) is a developmentally regulated transcription factor that recognizes a specific sequence CCCCAGGC or related sequences present in several promoter and enhancer gene regions including the promoters of the AP2 genes themselves. There are three different but highly homologous AP2 isoforms: alpha, beta, and gamma (ERF-1), encoded by three different retinoic acid-responsive genes. Each form of AP2 possesses the DNA binding domain (basic helix-span-helix motif) characteristic of this family of transcription factors. The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2639
AP2 alpha Antibody (AP2a 8G8/5) - BSA Free
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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TFAP2A
Entrez