AP2 alpha Antibodies
We offer AP2 alpha Antibodies for use in common research applications: Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Radioimmunodiffusion, Sandwich ELISA, Western Blot. Each AP2 alpha Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our AP2 alpha Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Human, Mouse, Primate, Rat, Sheep, Xenopus. Use the list below to choose the AP2 alpha Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our AP2 alpha polyclonal antibodies and browse our AP2 alpha monoclonal antibody catalog.
Alternate Names for AP2 alpha Antibodies
anti-AP2 alpha antibody, anti-TFAP2A antibody, anti-Activating enhancer-binding protein 2-alpha antibody, anti-Activator protein 2 antibody, anti-AP-2 transcription factor antibody, anti-AP-2transcription factor AP-2 alpha (activating enhancer-binding protein 2 alpha) antibody, anti-AP2-alpha antibody, anti-AP2TFAP-2alpha antibody, anti-BOFS antibody, anti-FLJ51761 antibody, anti-TFAP2 antibody, anti-transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) antibody, anti-transcription factor AP-2-alpha antibody
13 Results for "ap2-alpha" in Primary Antibodies
![Western Blot: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis was performed on whole cell extracts (30 ug lysate) of PC-3 (Lane1), A-431 (Lane2), Mouse Placenta (Lane3) and RAW 264.7 (Lane5).](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-3B5-Western-Blot-NB100-74359-img0011.jpg "Western Blot: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis was performed on whole cell extracts (30 ug lysate) of PC-3 (Lane1), A-431 (Lane2), Mouse Placenta (Lane3) and RAW 264.7 (Lane5).")
![Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) shows staining in Hela Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-3B5-Immunocytochemistry-Immunofluorescence-NB100-74359-img0008.jpg)
![Immunohistochemistry-Frozen: AP2 alpha Antibody (3B5) [NB100-74359] - AP2alpha staining on an E9.5 mouse pharyngeal mesoderm region. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-3B5-Immunohistochemistry-Frozen-NB100-74359-img0012.jpg)
![Western Blot: AP2 alpha Antibody (3B5) [NB100-74359] - Figure 1 illustrates Western blot detection of AP-2 from MCF7 cell lysate.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-3B5-Western-Blot-NB100-74359-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) shows staining in U251 Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-3B5-Immunocytochemistry-Immunofluorescence-NB100-74359-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) shows staining in MCF-7 Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-3B5-Immunocytochemistry-Immunofluorescence-NB100-74359-img0007.jpg)
![Immunohistochemistry-Paraffin: AP2 alpha Antibody (3B5) [NB100-74359] - Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-3B5-Immunohistochemistry-Paraffin-NB100-74359-img0009.jpg)
![Western Blot: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis using TFAP2A mAb against human TFAP2A (AA: 1-100) recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-7D2B5-Western-Blot-NBP2-52572-img0005.jpg "Western Blot: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis using TFAP2A mAb against human TFAP2A (AA: 1-100) recombinant protein. (Expected MW is 37 kDa)")
![Immunohistochemistry-Paraffin: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of paraffin-embedded TFAP2A tissues using endometrial cancer mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-7D2B5-Immunohistochemistry-Paraffin-NBP2-52572-img0004.jpg)
![Flow Cytometry: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of MCF-7 cells using TFAP2A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-7D2B5-Flow-Cytometry-NBP2-52572-img0002.jpg)
![Western Blot: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis using TFAP2A mAb against HEK293 (1) and TFAP2A (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-7D2B5-Western-Blot-NBP2-52572-img0006.jpg)
![Immunohistochemistry-Paraffin: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of paraffin-embedded ovarian cancer tissues using TFAP2A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-7D2B5-Immunohistochemistry-Paraffin-NBP2-52572-img0003.jpg)
![Flow Cytometry: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-7D2B5-Flow-Cytometry-NBP2-52572-img0001.jpg)
![AP2 alpha Antibody (7D2B5) [NBP2-52572] - Analysis using TFAP2A mouse mAb against Hela (1), MCF-7 (2), Cos7 (3), A431 (4), HCT116 (5), NIH/3T3 (6), and PC12 (7) cell lysate.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-7D2B5-NBP2-52572-img0007.jpg)
![ELISA: AP2 alpha Antibody (7D2B5) [NBP2-52572] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-7D2B5-ELISA-NBP2-52572-img0008.jpg)
![Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP1-90143] - Staining in human placenta and colon tissues using anti-TFAP2A antibody. Corresponding TFAP2A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-90143-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP1-90143] - Staining in human placenta and colon tissues using anti-TFAP2A antibody. Corresponding TFAP2A RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: AP2 alpha Antibody [NBP1-90143] - Analysis using Anti-TFAP2A antibody NBP1-90143 (A) shows similar pattern to independent antibody NBP2-38809 (B).](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Western-Blot-NBP1-90143-img0012.jpg)
![Western Blot: AP2 alpha Antibody [NBP1-90143] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Western-Blot-NBP1-90143-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody [NBP1-90143] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol, the Golgi apparatus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90143-img0007.jpg)
![Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP1-90143] - Staining of human colon shows low expression as expected.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-90143-img0008.jpg)
![Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP1-90143] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-90143-img0009.jpg)
![Dot Blot: AP2 alpha Antibody [NB100-56332] - Analysis of AP-2 in MCF-7 cell lysate with this antibody. A protein band of an approximate molecular weight of 55 kDa is detected.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Dot-Blot-NB100-56332-img0002.jpg "Dot Blot: AP2 alpha Antibody [NB100-56332] - Analysis of AP-2 in MCF-7 cell lysate with this antibody. A protein band of an approximate molecular weight of 55 kDa is detected.")
![Western Blot: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis using TFAP2A mAb against human TFAP2A (AA: 1-100) recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-1A10C5-Western-Blot-NBP2-52571-img0003.jpg "Western Blot: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis using TFAP2A mAb against human TFAP2A (AA: 1-100) recombinant protein. (Expected MW is 37 kDa)")
![Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis of Hela cells using TFAP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-1A10C5-Immunofluorescence-NBP2-52571-img0002.jpg)
![Flow Cytometry: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-1A10C5-Flow-Cytometry-NBP2-52571-img0001.jpg)
![Western Blot: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis using TFAP2A mAb against HEK293 (1) and TFAP2A (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-1A10C5-Western-Blot-NBP2-52571-img0004.jpg)
![ELISA: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-1A10C5-ELISA-NBP2-52571-img0005.jpg)
![Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP2-38809] - Staining in human placenta and colon tissues using anti-TFAP2A antibody. Corresponding TFAP2A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-38809-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP2-38809] - Staining in human placenta and colon tissues using anti-TFAP2A antibody. Corresponding TFAP2A RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: AP2 alpha Antibody [NBP2-38809] - Analysis using Anti-TFAP2A antibody NBP2-38809 (A) shows similar pattern to independent antibody NBP1-90143 (B).](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Western-Blot-NBP2-38809-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody [NBP2-38809] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38809-img0007.jpg)
![Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP2-38809] - Staining of human colon shows low expression as expected.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-38809-img0004.jpg)
![Immunohistochemistry-Paraffin: AP2 alpha Antibody [NBP2-38809] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-38809-img0005.jpg)
![Western Blot: AP2 alpha Antibody (PSH02-05) [NBP3-33043] - Western blot analysis of AP2 alpha on different lysates with Rabbit anti-AP2 alpha antibody (NBP3-33043) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: HepG2 cell lysate Lane 3: SiHa cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33043_rabbit-ap2-alpha-mab-psh02-05-288202412493482.jpg "Western Blot: AP2 alpha Antibody (PSH02-05) [NBP3-33043] - Western blot analysis of AP2 alpha on different lysates with Rabbit anti-AP2 alpha antibody (NBP3-33043) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: HepG2 cell lysate Lane 3: SiHa cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33043) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: AP2 alpha Antibody (PSH02-05) [NBP3-33043] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-AP2 alpha antibody (NBP3-33043) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33043) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33043_rabbit-ap2-alpha-mab-psh02-05-298202414304168.jpg)
![Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (PSH02-05) [NBP3-33043] - Immunocytochemistry analysis of HepG2 cells labeling AP2 alpha with Rabbit anti-AP2 alpha antibody (NBP3-33043) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AP2 alpha antibody (NBP3-33043) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33043_rabbit-ap2-alpha-mab-psh02-05-29202410234285.jpg)
![Flow Cytometry: AP2 alpha Antibody (A6/2/2) [NB600-202] - An intracellular stain was performed on Hek293 cells with AP2 alpha [A6/2/2] Antibody NB600-202AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-A6-2-2-Flow-Cytometry-NB600-202-img0001.jpg "Flow Cytometry: AP2 alpha Antibody (A6/2/2) [NB600-202] - An intracellular stain was performed on Hek293 cells with AP2 alpha [A6/2/2] Antibody NB600-202AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Western Blot: AP2 alpha Antibody (SR1826) [NBP3-22200] - Western blot analysis using NBP3-22200 in Hela cell lysate.](http://images.novusbio.com/fullsize/nbp3-22200_rabbit-ap2-alpha-mab-sr1826-57202315584843.jpg "Western Blot: AP2 alpha Antibody (SR1826) [NBP3-22200] - Western blot analysis using NBP3-22200 in Hela cell lysate.")
![Western Blot: AP2 alpha Antibody (PSH02-06) [NBP3-33044] - Western blot analysis of AP2 alpha on different lysates with Rabbit anti-AP2 alpha antibody (NBP3-33044) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33044) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33044_rabbit-ap2-alpha-mab-psh02-06-288202412493487.jpg "Western Blot: AP2 alpha Antibody (PSH02-06) [NBP3-33044] - Western blot analysis of AP2 alpha on different lysates with Rabbit anti-AP2 alpha antibody (NBP3-33044) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33044) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: AP2 alpha Antibody (PSH02-06) [NBP3-33044] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-AP2 alpha antibody (NBP3-33044) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33044) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33044_rabbit-ap2-alpha-mab-psh02-06-298202414333614.jpg)
![Immunocytochemistry/ Immunofluorescence: AP2 alpha Antibody (PSH02-06) [NBP3-33044] - Immunocytochemistry analysis of HepG2 cells labeling AP2 alpha with Rabbit anti-AP2 alpha antibody (NBP3-33044) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AP2 alpha antibody (NBP3-33044) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33044_rabbit-ap2-alpha-mab-psh02-06-29202410334070.jpg)
![Western Blot: AP2 alpha Antibody (8I8E4) [NBP3-16108] - Western blot analysis of extracts of various cell lines, using AP2 alpha Rabbit mAb (NBP3-16108) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-8I8E4-Western-Blot-NBP3-16108-img0001.jpg "Western Blot: AP2 alpha Antibody (8I8E4) [NBP3-16108] - Western blot analysis of extracts of various cell lines, using AP2 alpha Rabbit mAb (NBP3-16108) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")