![Flow Cytometry: AP2 alpha Antibody (A6/2/2) [NB600-202] - An intracellular stain was performed on Hek293 cells with AP2 alpha [A6/2/2] Antibody NB600-202AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-A6-2-2-Flow-Cytometry-NB600-202-img0001.jpg "Flow Cytometry: AP2 alpha Antibody (A6/2/2) [NB600-202] - An intracellular stain was performed on Hek293 cells with AP2 alpha [A6/2/2] Antibody NB600-202AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, Func, ICC/IF, IHC, CyTOF-ready |
| Clone | A6/2/2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Peptide to C-Terminal of AP2 alpha coupled to KLH. PNSHTDNNAKSSDKEEKHRK |
| Localization | Nuclear |
| Specificity | This antibody detects both the alpha and beta forms of AP2. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TFAP2A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Recommended for immunohistology using formal saline fixative with calcium on paraffin embedded specimens (10min microwave treatment required). This antibody is CyTOF ready. Positive control(s): Raji cells or breast carcinomas. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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