![Western Blot: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis using TFAP2A mAb against human TFAP2A (AA: 1-100) recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-1A10C5-Western-Blot-NBP2-52571-img0003.jpg "Western Blot: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis using TFAP2A mAb against human TFAP2A (AA: 1-100) recombinant protein. (Expected MW is 37 kDa)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, CyTOF-ready |
| Clone | 1A10C5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human AP2 alpha (AA: 1-100) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TFAP2A |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
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| Theoretical MW | 48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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| Publication using NBP2-52571 | Applications | Species |
|---|---|---|
| Sun X, Cui Z, Liang Y et al. One-stop assembly of adherent 3D retinal organoids from hiPSCs based on 3D-printed derived PDMS microwell platform Biofabrication 2023-04-11 [PMID: 36963105] |
Secondary Antibodies |
Isotype Controls |
Research Areas for AP2 alpha Antibody (NBP2-52571)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TFAP2A |
![Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis of Hela cells using TFAP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-1A10C5-Immunofluorescence-NBP2-52571-img0002.jpg "Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis of Hela cells using TFAP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.")
![Flow Cytometry: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-1A10C5-Flow-Cytometry-NBP2-52571-img0001.jpg "Flow Cytometry: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).")
![Western Blot: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis using TFAP2A mAb against HEK293 (1) and TFAP2A (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-1A10C5-Western-Blot-NBP2-52571-img0004.jpg "Western Blot: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Analysis using TFAP2A mAb against HEK293 (1) and TFAP2A (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/AP2-alpha-Antibody-1A10C5-ELISA-NBP2-52571-img0005.jpg "ELISA: AP2 alpha Antibody (1A10C5) [NBP2-52571] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).")
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