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ANP32A Antibody

Images

 
Western Blot: ANP32A Antibody [NBP2-49519] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: ANP32A Antibody [NBP2-49519] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & centrosome.
Immunohistochemistry: ANP32A Antibody [NBP2-49519] - Staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ANP32A Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GLEVLAEKCPNLTHLNLSGNKIKDLSTIEPLK
Predicted Species
Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANP32A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ANP32A Recombinant Protein Antigen (NBP2-49519PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ANP32A Antibody

  • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
  • acidic leucine-rich nuclear phosphoprotein 32 family member A
  • Acidic nuclear phosphoprotein pp32
  • cerebellar leucine rich acidic nuclear protein
  • I1PP2A
  • inhibitor-1 of protein phosphatase-2A
  • LANPPutative HLA-DR-associated protein I
  • Leucine-rich acidic nuclear protein
  • MAPMC15orf1
  • mapmodulin
  • PHAP1MGC150373
  • PHAPIMGC119787
  • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A
  • PP32
  • putative human HLA class II associated protein I

Background

Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ANP32A