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Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free

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 (2 Reviews)
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Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody (MM0019-26B10) [NB110-85464] - Angiopoietin 1 Antibody (MM0019-26B10) [NB110-85464] - Formalin fixed and paraffin embedded human placental tissue section is subjected to IHC with anti human Angiopoietin-1.
Immunohistochemistry-Paraffin: Angiopoietin-1 Antibody (MM0019-26B10) [NB110-85464] - Angiopoietin 1 Antibody (MM0019-26B10) [NB110-85464] - Formalin fixed and paraffin embedded human placental tissue section is ...read more

Product Details

Summary
Reactivity Hu, ChSpecies Glossary
Applications WB, IHC
Clone
MM0019-26B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-85464AF350
Alexa Fluor 405
NB110-85464AF405
Alexa Fluor 488
NB110-85464AF488
Alexa Fluor 532
NB110-85464AF532
Alexa Fluor 594
NB110-85464AF594
Alexa Fluor 647
NB110-85464AF647
Alexa Fluor 700
NB110-85464AF700
Alexa Fluor 750
NB110-85464AF750
Allophycocyanin
NB110-85464APC
Biotin
NB110-85464B
CoraFluor 1
NB110-85464CL1
DyLight 350
NB110-85464UV
DyLight 405
NB110-85464V
DyLight 488
NB110-85464G
DyLight 550
NB110-85464R
DyLight 594
NB110-85464DL594
DyLight 650
NB110-85464C
DyLight 680
NB110-85464FR
DyLight 755
NB110-85464IR
FITC
NB110-85464F
HRP
NB110-85464H
Janelia Fluor 525
NB110-85464JF525
Janelia Fluor 549
NB110-85464JF549
Janelia Fluor 585
NB110-85464JF585
Janelia Fluor 635
NB110-85464JF635
Janelia Fluor 646
NB110-85464JF646
Janelia Fluor 669
NB110-85464JF669
PE
NB110-85464PE
PerCP
NB110-85464PCP
mFluor Violet 450 SE
NB110-85464MFV450
mFluor Violet 500 SE
NB110-85464MFV500
mFluor Violet 610 SE
NB110-85464MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NB110-85464-20ug
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Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free Summary

Description
PLEASE NOTE: 0.02mg sample size is provided in reconstituted format and the 0.1mg size is provided lyophilized. (Please see reconstitution instuctions).
Immunogen
Human recombinant human Angiopoietin-1
Specificity
No cross reactivity with human Angiopoietin-2.
Isotype
IgG2
Clonality
Monoclonal
Host
Mouse
Gene
ANGPT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Western Blot 1:1000-1:2000
Application Notes
For use in FFPE tissue, our recommended antigen retrival-treat the paraffin section with 20ug/ml Proteinase K (in PBS) for 15-25min. Angiopoietin-1 antibody validated for IHC-FR from a verified customer review.
Control
Angiopoietin-1 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 3
using
NB110-85464 in the following applications:
Publications
Read Publication using NB110-85464.

Reactivity Notes

Chicken reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS. 20 ug size is provided in liquid form, PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free

  • AGP1
  • AGPT
  • Ang1
  • ANG-1
  • angiopoietin 1
  • Angiopoietin-1
  • ANGPT1
  • KIAA0003

Background

Angiopoietin 1 is one of the Angiopoietin (Ang) family of growth factors. It binds and activates the TIE2 receptor tyrosine kinase by inducing its tyrosine phosphorylation. It is implicated in endothelial developmental processes later and distinct from that of VEGF. The structure of angiopoietin-1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Angiopoietin 1 appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It also mediates blood vessel maturation/stability and it may play an important role in the heart early development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Angiopoietin-1
Antibody (MM0019-26B1 ...
NB110-85464
Angiopoietin-1 Antibody (MM0019-26B10) - Azide and BSA Free
Species: Hu, Ch
Applications: WB, IHC

Publications for Angiopoietin-1 Antibody (NB110-85464)(1)


Reviews for Angiopoietin-1 Antibody (NB110-85464) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NB110-85464:
Filter by Applications
IF
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Angiopoietin-1 NB110-85464
Enlarge 		3
reviewed by:
Bryan Tinsley
 IF Other 08/26/2014
View

Summary

ApplicationImmunofluorescence
SpeciesOther
Immunohistochemistry-Frozen Angiopoietin-1 NB110-85464
Enlarge 		3
reviewed by:
Corey .
 IHC-Fr Human 07/12/2013
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Angiopoietin-1 Antibody (NB110-85464). (Showing 1 - 1 of 1 FAQ).

  1. How do you dissolve the lyophilized Ab? Water or PBS? The datasheet for NB110-85464 just says "Buffer: Lyophilized" - - in Water? Buffer? Concentration of Buffer? NB110-85467 says Lyophilized from a solution of PBS - again how to dissolve the Ab - which PBS and what concentration?
    • The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). 100 ug antibody is lyophilized from 40 ul of 1 x PBS. We suggest reconstituting the antibody with 500 ul sterile 1 x PBS (or to be perfect, with 40 ul H2O plus 460 ul PBS), to give a final concentration of 200 ug/ml. Alternatively, you may reconstitute the antibody in 40 ul H2O if a small volume is preferred.

Control Lysate(s)

Angiopoietin-1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Research Areas for Angiopoietin-1 Antibody (NB110-85464)

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Blogs on Angiopoietin-1

There are no specific blogs for Angiopoietin-1, but you can read our latest blog posts.
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Contact Information

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Calculators

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Recent Reviews

3
5
0
4
0
3
2
2
0
1
0
Immunofluorescence Angiopoietin-1 NB110-85464
Enlarge
 Bryan Tinsley
08/26/2014
Application: IF
Species: Other
read this review
Immunohistochemistry-Frozen Angiopoietin-1 NB110-85464
Enlarge
 Corey .
07/12/2013
Application: IHC-Fr
Species: Human
read this review

Bioinformatics

Gene Symbol ANGPT1
Entrez
Uniprot