![Western Blot: AlphaB Crystallin/CRYAB [p Ser45] Antibody [NB120-5598] - Analysis of recombinant bovine phospho-alpha-B crystallin (Ser45). Lane 1 is phospho-alpha-B crystallin (Ser45) and lane 2 is unphosphorylated alpha-B crystallin.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-[p-Ser45]-Antibody-Western-Blot-NB120-5598-img0004.jpg "Western Blot: AlphaB Crystallin/CRYAB [p Ser45] Antibody [NB120-5598] - Analysis of recombinant bovine phospho-alpha-B crystallin (Ser45). Lane 1 is phospho-alpha-B crystallin (Ser45) and lane 2 is unphosphorylated alpha-B crystallin.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, IHC, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | LYOPH |
| Immunogen | Synthetic phopshopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin. |
| Modification | p Ser45 |
| Localization | Cytoplasmic |
| Specificity | This does not detect the unphosphorylated form of the protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CRYAB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Detects an approx. 20 kDa representing recombinant bovine phospho-alpha-B crystallin (Ser45). IF: Staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute to desired concentration. |
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| Publication using NB120-5598 | Applications | Species |
|---|---|---|
| Ueda S, Nishihara M, Hioka Y et al. Polo-Like Kinase 2 Plays an Essential Role in Cytoprotection against MG132-Induced Proteasome Inhibition via Phosphorylation of Serine 19 in HSPB5 International Journal of Molecular Sciences 2022-09-24 [PMID: 36232565] (Western Blot, Immunocytochemistry/ Immunofluorescence) | Western Blot, Immunocytochemistry/ Immunofluorescence |
Secondary Antibodies |
Isotype Controls |
Research Areas for AlphaB Crystallin/CRYAB Antibody (NB120-5598)Find related products by research area.
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